光度法测铁离子浓度(English).docVIP

Determination of iron by Phenanthroline photometric method Ⅰ.Principle Abserption spectrophotometric determination of trace iron is one of the commonly used method. At pH = 1.5 ~ 9.5 under the conditions, Fe2 + ion and phenanthroline (Phen) generated very stable orange-red complex with Fe (Phen)32 +: ?Stability of this complex lg K = 21.3, molar absorption coefficient ε = 1.1 × 104 L / mol ? cm. Hair color before the first use of hydroxylamine hydrochloride to Fe3 + ions reduced to Fe2 + ions, the reaction is as follows: ?????????????4 Fe3 + + 2 NH2OH = 4 Fe2 + + N2O + H2O + 4H + Determina