- 1、本文档共5页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
查看更多
光度法测铁离子浓度(English)
Determination of iron by Phenanthroline photometric method
Ⅰ.Principle
Abserption spectrophotometric determination of trace iron is one of the commonly used method. At pH = 1.5 ~ 9.5 under the conditions, Fe2 + ion and phenanthroline (Phen) generated very stable orange-red complex with Fe (Phen)32 +: ?Stability of this complex lg K = 21.3, molar absorption coefficient ε = 1.1 × 104 L / mol ? cm. Hair color before the first use of hydroxylamine hydrochloride to Fe3 + ions reduced to Fe2 + ions, the reaction is as follows: ?????????????4 Fe3 + + 2 NH2OH = 4 Fe2 + + N2O + H2O + 4H + Determination, the control solution acidity is more appropriate in the pH = 3-9 with high acidity, the reaction is slow; acidity is too low then the hydrolysis of Fe3 + ions affect the color. Bi3 +, Cd2 +, Hg2 +, Ag +, Zn2 + and other ions with the reagent generated precipitation, Ca2 +, Cu2 +, Ni2 + and other ions form a color complex, so when these ions coexist, it should be noted their interference. Ⅱ.the instruments and reagents 1. UV-2100 spectrophotometer, 50mL volumetric flask of 8;2. Iron standard solution (100 μg / mL);3. Iron standard solution (10 μg / mL);4. Phenanthroline (1.5 g / L);5. Hydroxylamine hydrochloride (100 g / L) ;6. NaAc (1mol / L);7. NaOH (1mol / L);8. HCl (6mol / L);9. Iron sample test solution. Ⅲ.procedures 1. Absorption curve of production and the choice of measurement wavelength Lessons with a pipette 0.0 mL and 1.0 mL iron standard solution (100 μg / mL) were injected into two 50mL volumetric flask, add 1 mL of the hydroxylamine hydrochloride solution, shake well. Then add 2 mL Phen, 5 mLNaAc, diluted with water to the mark, shake well. 10 min after placement, with the 1cm cuvette to reagent blank (ie, 0.0 mL iron standard solution) as reference solution, between the 470-560nm, measured once every 10 nm absorbance A, at the maximum absorption peak near measured once every 2 nm A. In the graph paper at the wavelength λ is the horizontal a
文档评论(0)