胆固醇代谢过程.doc

Fig. 2. Proposed pathway for cholesterol degradation under aerobic conditions. Cholest-4-en-3-one or any of the subsequent metabolites from degradation of the side-chain up to (and including) AD may undergo a dehydrogenation reaction to introduce a double bond in the position 1, leading to compound cholest-1,4-diene-3-one in the case of cholest-4-en-3-one, or to the corresponding 1,2-dehydro derivatives for other molecules. The side-chain degradation of this compounds will be identical to that of the cholest-4-en-3-one to the common intermediate 9a-hydroxyandrosta-1,4-diene-3,17-dione. The microorganisms from which enzymes implicated in different steps are indicated by numbers. Numbers in brackets are assigned arbitrarily to facilitate the compound identification in Fig. 3. Proposed b-oxidation-like reactions for cholesterol side-chain degradation. The Fad proteins have been assigned according to the nomenclature of the E. coli genes involved in the b-oxidation of fatty acids. LiuE is the name assigned to 3-hydroxy-3-methylglutaryl-coenzymelyases. Fig. 4. Organization of the main gene clusters implied or suggested to be involved in the degradation of cholesterol in M. smegmatis mc2155.The identity number for each MSMEG gene is indicated within the arrows. The name of some genes of interest is written above them. Numbers below genes indicate the number of bp between adjacent genes; numbers in brackets indicate separation and numbers in parentheses indicate overlap. Numbers above diagonal lines indicate the genomic position in kb. Orange: mce cluster. Green: genes suggested and/or proved to participate in the side-chain degradation. Blue: genes suggested and/or proved to participate in the central or lower catabolic pathway. Yellow: genes coding the transcriptional repressors KstR and KstR2. Genes surrounded by a dashed line are controlled by KstR2, the rest of the genes showed in this figure are controlled by KstR (except for MSMEG_5905, 5909, 5910, 5912, 5916,