canoco软件使用实例-CCA 分析.docVIP

Canoco 软件操作的简单教程 步骤： 1． 打开excel文档，如下图所示，包含环境变量（12个）和生物因子（15种C源利用性）。共是8个处理，每个处理三个重复，总共是24个土壤样品。Potential: potentially mineralizable organic C pools; SOC, soluble organic C extracted by 2 M KCl ; SON, soluble organic N extracted by 2 M KCl; soil moisture content; TC, Total C; TN, Total N; WHC, water holding capacity; MBC, microbial biomass C; MBN, Microbial biomass N. The 15 C sources were chosen to cover a range of low molecular weight compounds (including carbohydrates, amino acids and carboxylic acids) that are commonly in root exudates (Campbell et al., 2008). The numbers from 1 to 16 correspond to represent L-alanine, L-arginine, citric acid, D-galactose, amino butyric acid, malic acid, oxalic acid, 3,4-OH benzoic acid, L-arabinose, L-cysteine, D-fructose, D-glucose, L-lysine, N-acetyl glucosamine, D-trehalose, water, respectively. ?选 分别保存文件为environ.dta (dta是canoco默认的样式)和 species.dta. 文件形式如下图。 2. 打开canoco 软件—new project，得到下面的界面。 3．点下一步后，有三个brown窗口，前2个分别选择 environ.dta 和species.dta. 第三个选择软件自带合适的处理方法（如CCA和RDA等）。本文是选用RDA分析。 4．其他还有一些窗口，但是问题不大，这里就不多说了。直接进入下一步，点击analyze，如下图所示。完成后点击canodraw。 5．点击create 窗口，如下图所示。得到最后的结果。 结果的解释： The relationships between environmental factors and microbial metabolic profiles were as shown above. Plots can be interpreted quantitatively using the length of the arrow to indicate how much variance was explained by that factor; the direction of the arrows for individual environmental factor indicating an increasing weighting of that factor. The soil sample arrows pointing in approximately the same direction as the environmental arrows indicate a high positive correlation (the longer the soil sample arrow, the stronger the relationship; Kennedy et al., 2004). Among all environmental factors, C0 exhibited the greatest influence on the soil samples (P0.01) followed by pH and soil moisture and SON content. What is driving changes? Previous studies have suggested that RDA was a suitable method for quantify the relationship between environmental f