发酵罐批式半连续培养发酵制氢试验.pdfVIP

第34 卷 第12H 期 Vol. 34 No.12H 第12H 期 Dec. 2011 2011 年12 月 Environmental Science Technology 陈璐圆，钱春香.发酵罐批式半连续培养发酵制氢试验探索[J]. 环境科学与技术, 2011, 34(12H):29- 32. Chen Lu- yuan, Qing Chun- xiang. The exploration of hydrogen production by eermentation in fermenter with batch and semi- continuous culture[J]. Environmental Science Technology, 2011, 34(12H):29- 32. 发酵罐批式半连续培养发酵制氢试验探索 陈璐圆，钱春香* （东南大学材料科学与工程学院，江苏 南京 211189） 摘 要：在10 L 的发酵罐中，按照糖蜜的主要成分配制发酵培养基，考察和分析了批式、半连续发酵方式下，环境因素对固定化陶 粒中厌氧菌发酵产氢的影响。结果表明，批式发酵产氢以糖蜜为底物的产氢得率、氢浓度和产氢速率分别为1.40 mol/mol 蔗糖，53%和 240 mL/(L ·h) 。可以在工业制氢中作为糖蜜快速发酵制氢的方法。 关键词：发酵产氢；细菌固定化；产氢能力；半连续产氢 中图分类号：X703 文献标志码：A doi ：10.3969/j.issn.1003-6504.2011.12H.007 文章编号：1003-6504(2011)12H-0029-04 The Exploration of Hydrogen Production by Fermentation in Fermenter with Batch and Semi-continuous Culture CHEN Lu-yuan, QIAN Chun-xiang* （School of Materials Science and Engineering, Southeast University, Nanjing 211189, China） Abstract ：The impact of the environmental factors on hydrogen production is studied and analyzed with the batch, semi- continuous fermentation in 10 L fermentation tank. Anaerobic bacteria is immobilized inside the ceramic, and the fermentation medium is in accordance with the main component of molasses. The results show that hydrogen production by batch fermentation of molasses as substrate is much more efficient than by semi -continues culture. And the yield, hydrogen concentration and hydrogen production rates were 1.40 mol/mol sucrose, 53% and 240 mL/(L ·h) sepa