染色体论文：探讨染色体Cd结构及CENP变化与非整倍体形成的关系.docVIP

染色体论文：探讨染色体Cd结构及CENP变化与非整倍体形成的关系 【中文摘要】研究流产绒毛染色体非整倍体及其双亲染色体着丝粒点(Cd)结构变异和着丝粒蛋白(CENP)表达情况，探讨子代非整倍体形成及其双亲染色体Cd结构变异和CENP表达的关系。方法：以非整倍体流产绒毛及其双亲为研究组，以正常绒毛及其双亲为对照组。应用染色体G显带技术、改良的着丝粒点-核仁组织区(Cd - NOR)同步银染方法及免疫组化技术，对每例标本进行染色体核型分析、Cd变异及CENP检测。结果：①两组流产绒毛染色体的Cd变异有显著差异，研究组中的流产绒毛染色体Cd变异明显高于对照组（t＝14.785，p﹤0.05）。②研究组中的流产绒毛染色体Cd变异高于双亲外周血染色体，差异有统计学意义,（t＝②14.965 p②﹤0.05，t＝③15.371，p③﹤0.05）。③对照组中的流产绒毛染色体Cd变异与其双亲外周血染色体比较，差异无统计学意义（t④＝2.463 p④﹥0.05，t＝⑤2.156 p⑤﹥0.05）④两组流产绒毛染色体中均有CENP的表达，且研究组(灰度值：165.53±4.81)显著低于对照组(灰度值：160.87±4.62)( t＝3.012，p﹤0.05)。⑤研究组非整倍体绒毛染色体中CENP含量低于其父方，差异有统计学意义（t①＝-4.279，p①﹤0.05），而与母方的差异无统计学意义（t②＝-1.927,p②﹥0.05)。⑥对照组正常绒毛染色体CENP含量低于其父方，差异有统计学意义（t③＝-3.008,p③﹤0.05),而与母方的差异无统计学意义(t④＝-1.234 p④﹥0.05)。⑦CENP的含量与Cd结构变异呈负相关。结论：Cd变异、CENP含量降低可能是染色体着丝粒不分离造成非整体形成的原因之一，且CENP的含量与其母亲具有一定的同源性，染色体中CENP的含量与Cd变异呈负相关，即Cd变异数/细胞数越高，CENP的含量越少。 【英文摘要】objectives:In order to explore the relation between embryos of aneuploidoccurrence and their parents ,this paper investigated the variations of centromeric dots (Cd)and CENPs of them . Methods: there are two groups，the test group are chromosome ofabortioning embryos which were been diagnosed had stopped growth and the chromosomeof their parents, the compare group are the embryos which are chromosome of normal and thechromosome of their parents, The peripheral blood lymphocyte chromosome specimens weremade with the routine chromosomal technique ,G- band were developed. A newly - modifiedsynchronous ,Ag - staining technique ,was used to get both Cd. And to use the technique ofImmunhitehmisty to get the CENPs .all the cases have whole data of G-bandtechnique.Results :①The variations of centromeric dots (Cd) of the test group and the comparegroup were different,the test group was higher than the compare group.（t＝14.785，p﹤0.05）.②The aneuploid embryos group of testing was higher than the their maternal group andtheir paternal group（t＝②14.965,p②﹤0.05;t＝③15.371,p③﹤0.05 ).③The variations ofcentromeric dots (Cd) in the compare group were no significant differ