放线菌分离培养基筛选及杂菌抑制方法研究_cropped.doc

 放线菌分离培养基筛选及杂菌抑制方法研究 3 司美茹 薛泉宏 来航线 ( 西北农林科技大学资源环境学院 杨凌 712100) 摘要 : 采用平板涂抹分离法研究了培养基种类 、土壤样品加热预处理及化学抑制剂对放线菌分 离效果的影响 。结果表明 : 高氏 1 号琼脂培养基 ( GA) 和秸秆腐解物琼脂培养基 ( SDSA) 的分 离结果可以反映土壤中放线菌的基本状况 ; 120 ℃×110h 加热预处理土样能促进放线菌孢子萌 发 , 增加放线菌的分离数量和种类 。供试土样经 120 ℃×110h 加热预处理后放线菌的数量和类 型较对照处理分别增加了 515 %～5419 %和 1215 %～100 % , 但加热处理对减少细菌数量作用不 明显 ; 在放线菌分离培养基中同时加入 K2 Cr2 O7 75μgΠmL 与 1～3μgΠmL 青霉素 , 细菌数量减少 , 1 号土样在 SDSA 培养基上放线菌数量 ( ×106 个Πg) 和种类 (种) 分别较仅加入 75μgΠmL K Cr O 2 2 7 处理减少 0～5818 %和 0～1812 % ; 链霉素不能用作分离放线菌时的细菌抑制剂 。 关键词 : 放线菌 , 微生物资源 , 放线菌分离 , 培养基 中图分类号 : Q931331 文章编号 : 025322654 (2004) 0220061205 文献标识码 : A STUDIES ON SEL ECTION OF THE ISOLATION MEDIUM FOR ACTINOMYCETES AND INHABITION METHODS TO MISCELLANEOUS MICROORGANISM SI Mei2Ru XUE Quan2Hong LAI Hang2Xian ( College of Resources and Environmental Scienc , Northwest Sci2Tech University of Agriculture and Forestry , Yangling 712100) Abstract : The influence of medium variety、thermal treatment of the soil samples and inhibitor to the isolation effect of acti2 nomycetes was studied by plate paint isolation methods1The results showed that : ①The isolation results of gauze No11 ( GA) and straw decay substance agar ( SDSA) might reflect basic station of soil actinomycetes1 ②Thermal treatment (120 ℃×110h) in soils might promote actinomycetes spore sprouting and increase the quantity and variety of actinomyce2 tes1 The quantity and varieties of actinomycetes in the Soils that were treated with 120 ℃×110h were increased by 515 %～ 5419 % and 1215 %～100 % than the check of none2thermal treatment respectively1 But thermal treatment had no effect on reduce the quantity of bacteria1 ③The quantity of bacteria had significant reduction by adding 75μgΠmL K2 Cr2 O7 and 1～ 3μgΠmL penicillin at same time to the isolation medium , and had little effect on the quantity and varieties of actinomycetes1 Actinomycetes quantity ( ×106 cfuΠg) and variety of the treating of 75μgΠmL K2 Cr2 O7