干旱胁迫下刚毛柽柳消减文库的构建及分析-植物研究-东北林业大学.pdfVIP

25 　1 　　　　　　　　　　　　　　　　　　　　　　 2005 　1 Vol.25　No.1　　　　　　　　　　　　BULLETIN OF BOTANICAL RESEARCH Jan., 　2005 1, 2 2 2 2 刘桂丰 　侯英杰 　王玉成 　褚延广 (1., 　100083) (2., 　150040) 　　以干旱胁迫下的刚毛柽柳根部组织 DNA 为tester,正常生长的刚毛柽柳根部组织 DNA 为driver,利用抑制性消减杂交技术(SSH )建了干旱胁迫下刚毛柽柳根部组织的消减文库。文库 克隆的重组率为95 %,插入片段大部分集中在250 ～600 bp之间。通过对文库阳性克隆的随机测 序, 获得了如Mn-SOD、myb 相关蛋白、锌指蛋白等 17 种与干旱胁迫相关的基因, 它们涉及了植物 的渗透调节、信号传递、转录调控、活性氧清除等方面。所得EST 序列已被GenBank 收录。实验为 抗逆基因克隆和系统研究干旱胁迫下柽柳根部基因的表达奠定了基础。 　干旱胁迫;刚毛柽柳;抑制性消减杂交;表达序列标签 Construction and analysis of Tamarix hispida suppression subtractive hybridization library under drought stress 1,2 2 2 2 LIU Gui-Feng 　HOU Ying-Jie 　WANG Yu-Cheng 　CHU Yan-Guang (1.S hool of Biologi al S ien es and Biote hnology, Beijing Forestry University, Beijing 　100083) (2.Forest College Northeast Forestry University, Harbin　150040) Abstract 　Using DNA from Tamarix hispida roots treated with drought stress as tester and DNA from Tamarix hisp ida roots in normal growth as driver, suppression subtra tive hybridization (SSH)was employed to onstru t DNA subtra ted library.In the library, the rate of re ombination was 95%, the size of inserts was 250 ～600 bp.17 drought stress-asso iated genes were obtained by DNA sequen ing the positive lones pi ked randomly,su h as Mn-SOD myb-related protein zin finger protein, et .These genes in luded osmoti regulator signal omponent regulatory protein and antioxidant enzyme.GenBank had a epted all the se- quen es.The resear h had established a basis for loning stress-resistan e genes and further studying genes expression in Tamarix hisp