rho／rock信号通路在腹膜透析大鼠腹膜纤维化中的作用及法舒地尔的干预效应.docVIP

精品论文 参考文献 Rho／ROCK信号通路在腹膜透析大鼠腹膜纤维化中的作用及法舒地尔的干预效应 孟绪初1 甘平2（通讯作者） 　　（1贵州医科大学研究生学院 贵州 贵阳 550004） 　　（2贵州医科大学附属乌当医院肾内科 贵州 贵阳 550018） 　　【摘要】 目的：探讨Rho／ROCK信号通路在腹膜透析大鼠腹膜纤维化中的可能机制。方法:将30只雄性SD大鼠随机分为四组：对照组，每日腹腔注射生理盐水25ml；模型组，每日腹腔注射4.25%的腹透液25ml；Fasudil组，每日腹腔注射含法舒地尔(Fasudil)浓度为10mg /Kg?d的4.25%腹透液25ml。实验第28天，取壁层腹膜组织行HE染色，并测定壁层腹膜ROCK-1的表达情况及SOD、MDA含量。结果：与模型组比较，Fasudil组不同程度腹膜厚度减少、胶原纤维沉积减轻，腹膜组织ROCK-1表达减少(Plt;0.05)，MDA含量降低(Plt;0.05)，SOD活性增加(Plt;0.05)。结论：腹膜透析液激活了腹膜透析大鼠腹膜Rho／ROCK信号通路，促进腹膜组织氧化应激损伤，导致了腹膜纤维化的发生，ROCK抑制剂法舒地尔能通过抑制此通路减少腹膜的氧化应激反应，进而延缓腹膜纤维化进展。 　　【关键词】 腹膜透析；腹膜纤维化；氧化应激；Rho／ROCK信号通路 　　【中图分类号】R459.5 【文献标识码】A 【文章编号】2095-1752（2016）08-0059-03 　　Rho/ROCK signaling pathway in pd rat peritoneal fibrosis, the effect of the intervention effect of shu, and the method 　　Meng Xuchu 1 GanPing 2.1Guizhou GuiZhou GuiYang medical University graduate school of 550004,China.2Wu when Hospital affiliated to medical University renal medicine in guizhou GuiZhou GuiYang 550018,China 　　【Abstract】Objective To study the Rho/ROCK signaling pathway in the possible mechanism of pd rat peritoneal fibrosis. Methods 30 male SD rats were randomly divided into four groups: control group, the daily intraperitoneal injection of saline 25 ml; Model group, the daily intraperitoneal injection of 4.25% of the liquid to drain 25 ml; By intraperitoneal injection of Fasudil group, daily with shu to farmar (Fasudil) concentration of 10 mg/Kg, 4.25% of the d to drain fluid 25 ml. Experiment 28 days, parietal layer and organizational line HE staining, and the determination of parietal layer and the expression of ROCK - 1 and SOD, MDA content. Results Compared with model group, different Fasudil group peritoneal thickness reduce, collagen fibers deposition reduction, reduced peritoneal organization ROCK - 1 expression (P lt; 0.05), lower MDA content increased (P lt; 0.05), SOD activity (P lt; 0.05). Conclusion Peritoneal dialysate activates the pd rat peritoneal Rho/ROCK signalin