Ethanol fermentation in an immobilized cell reactor using Saccharomyces cerevisiae外语英文电子书.pdfVIP

Bioresource Technology 92 (2004) 251–260 Ethanol fermentation in an immobilized cell reactor using Saccharomyces cerevisiae Ghasem Najafpour *, Habibollah Younesi, Ku Syahidah Ku Ismail School of Chemical Engineering, Engineering Campus, Universiti Sains Malaysia, Sri Ampangan, Nibong Tebal S.P.S., 14300 Pulau Pinang, Malaysia Received 2 January 2003; received in revised form 22 September 2003; accepted 23 September 2003 Abstract Fermentation of sugar by Saccharomyces cerevisiae, for production of ethanol in an immobilized cell reactor (ICR) was suc- cessfully carried out to improve the performance of the fermentation process. The fermentation set-up was comprised of a column packed with beads of immobilized cells. The immobilization of S. cerevisiae was simply performed by the enriched cells cultured media harvested at exponential growth phase. The fixed cell loaded IC Rwas carried out at initial stage of operation and the cell was entrapped by calcium alginate. The production of ethanol was steady after 24 h of operation. The concentration of ethanol was affected by the media flow rates and residence time distribution from 2 to 7 h. In addition, batch fermentation was carried out with 50 g/l glucose concentration. Subsequently, the ethanol productions and the reactor productivities of batch fermentation and immobilized cells were compared. In batch fermentation, sugar consumption and ethanol production obtained were 99.6% and 12.5% v/v after 27 h while in the ICR, 88.2% and 16.7% v/v were obtained with 6 h retention time. Nearly 5% ethanol production was achieved with high glucose concentration (150 g/l) at 6 h retention time. A yield of 38% was obtained with 150 g/l glucose. The yield was improved approximately 27% on IC Rand a 24 h f