应用生物芯片技术研究人参对衰老小鼠脑组织基因表达谱的影响研究.pdfVIP

第19卷第5期 传感技术学报 V01．19．No．5 A舳∞m蛐吣 2006年10月 嘣愀JOU心札∞删R8 Oct．2006 Jibe嗽西№mOme h咖砌sed D|i聊嘟．眦姗疆鳟笔MIDe m恤础IIj小舶唧 YE Yu-Bo Fang+，3qdANV％i—zheng，WANG 710072，China) (MEMS／NEMSLaboratory，NorthwesternPolytechnicalUniversity，Xi’nn subacutemodel themolecularmechanismofPana)【．Methods：The Abstract：Objective：Toinvestigate aging miceinduced were mRNAderivedfromthe and byD-galactoseapplied．Equalquantity experimentalgroup thecontrol werelabeledwith and as mixed group cy3一dUTPcy5一dUTPproberespectively．Theprobes werethen withBiostarM-20scDMA was GenePix microarray．Theimageanalyzedby hybridized acquired werescreened areall encoded Pr03．0．Results：13differentiallyexpressedgenes out．Theyprotein genes． thatPanaxindeedhaveeffectson of modelmice Conclusion：It’Sproved geneexpressionprofileaging brain and bethe ofthe effectofPanaK tissue．NckaplAtp5algenemay targetgenes anti—aging Keywords：Bio—MEMS；Panax；anti—aging；cDNAmicroarray EEAoC：7230j 向 叶 芳+，苑伟政，王宇波 (西北工业大学微／纳米系统实验室，西安710072) 摘 要：目的：利用生物芯片技术分析人参对衰老小鼠脑组织基因表达谱的影响，探讨人参作用机理研究方法．方法：20只雌 性小鼠用D一半乳糖诱导为亚急性衰老模型小鼠，并随机分为两组：实