羟基自由基快速杀灭典型水华藻的研究.pdfVIP

中国环境科学 2017,37(7) ：2633~2638 China Environmental Science 羟基自由基快速杀灭典型水华藻的研究 丁丽飞 1,李海燕 1, 白敏冬 1*,郑 武 1,郭 枫 1,张芝涛 2* (1.厦门大学环境与生态学院,海洋生物资源开发利 用协同创新中心,福建 厦门 361102 2.大连海事大学环境工程研究所物理系,辽宁 大连 116026) 摘要： 以典型水华藻铜绿微囊藻、针杆藻和四尾栅藻为研究对象,利用大气压强电离放电高效生成的羟基自由基(•OH)对 3 种藻进行杀灭. 采用荧光染色、流式细胞仪和光合活性等生物学方法,确定⋅OH 杀灭的阈值浓度和时间,并观察细胞形态变化.结果表明,当混合藻中铜绿微 4 4 4 囊藻、针杆藻和四尾栅藻的初始藻密度分别为 19.5×10 、21.8×10 和 4.90×10 cells/mL 时, ⋅OH 杀灭的阈值浓度为 1.07mg/L,致死时间为 4.5s;形态观察结果表明,处理后各种藻的形态是完整的,无内溶质溢出.因此,采用•OH 可实现高效快速杀灭水华藻,有效保障饮用水安全. 关键词：羟基自由基；水华藻；致死阈值；暴露时间 中图分类号：X52 文献标识码：A 文章编号：1000-6923(2017)07-2633-06 1 1 Research on the rapid inactivation of typical algae blooms by hydroxyl radical. DING Li-fei , LI Hai-yan , BAI min-dong1* 1 1 2* , ZHENG Wu , GUO Feng , ZHANG Zhi-tao (1.College of Environment Ecology, Xiamen University, Xiamen 361102, China 2.Environmental Engineering Institute, Dalian Maritime University, Dalian 116026, China). China Environmental Science, 2017,37(7)：2633~2638 Abstract ：Algae blooming in water sources breaks out increasingly and seriously threatened the water supply safety. Bench scale tests were conducted to study the effects of ⋅OH generated from strong ionization discharge and high pressure water jet cavitation on cell density, cell integrity and photosynthetic capacity of 3kinds of typical freshwater algae. Algae species including Microcystis aeruginosa, Synedra sp., and Scenedesmus quadricuauda were respectively prepared at concentrations of 4 4 4 19.5×10 , 21.8×10 and 4.90×10 cells/mL, and the cell integrity was