银杏叶提取物影响臂丛撕脱后运动神经元NOS表达及其存活.PDFVIP

Chinese Jo rnal of Pathophysiology 2003, 19( 9) : 1231- 1233 1231 [ ] 1000- 4718(2003) 09- 1231- 03 NOS * 1 1 1 1 1 2 李方澜, 周丽华 , 袁群芳 , 何宏文, 姚志彬 , 吴武田 ( 1, 510080; 2, ) [ ] : EGb761 NOS : Sprag e- Dawley 80 , C5T1 , 1 mL EGb761, 25 mg kg- 1 d- 1; 1 2 4 6 , C7 NADPH- d , NOS , : NOS 1 , 2 , 4 - 6 4 - 6 EGb761 NOS , : EGb761 NOS , [ ] ; ; ; [ ] R3382 [ ] A [ 1- 4] ( nitric oxide synthase, NOS) EGb761 ( nitric oxide, NO) , (, 0866- 9701) NOS, 25 mg kg- 1 d- 1 , , 1 mL ip , NOS , 10 , NOS 12 4 6 [ 1- 4] , NO , 3 NADPH- d , 1% (40 mg/ kg, ip) [5] , 4% ( extract of Ginkgo biloba, EGb761) PBS C7 [6] , C7 , 4 h , , EGb761 30% 4 ! , , EGb761 NOS , 40 m, 3 1, 001 mol/ L PBS( pH 7. 4) ( Nicotinamide adenine din cleotide phosphate- diaphorase, - NADPH 1 - d) NOS [ 1- 4] NADPH- d Sprag e- Dawley 80 , 200- 300 NADPH- d( Sigma ) 10 mg; g, 12 h ( ) 25 mg37 ! , , 1- 2 h , 001 mol/L PBS( pH 7. 4) 3 EGb761 , , 1%3- 5 min, 70% 10 , , 2 EGb761 4 20 [ ] 2003- 03- 06 [ ] 2003- 04- 25 , 10 , , * [] (No. 2002C30111) , Tel:020- E- mail: zlh a62@yahoo. com. cn 100% , NOS 1232