U251人神经胶质瘤细胞.PDFVIP

U251 人神经胶质瘤细胞 说明书修订日期 Cat Number：KG050 For Research Use Only 一、组成 组 份 KG050 细胞一瓶 25cm2 细胞说明书 1份 细胞培养注意事项 1份 二、客户自备试剂 1、PBS (凯基货号：KGB5001) 2、Complete growth medium (凯基货号：KGM12800) 3、0.25% （W/V）Trypsin-0.53mM EDTA(凯基货号：KGY0012) 4、FBS 三、细胞简介 Growth Properties: adherent Keygen No KG050 Organism: Homo sapiens (human) Morphology: Fibroblast-like Source: Brain Image: Propagation: Complete growth medium: DMEM(High glucose)+10%FBS+P/S Temperature:37.0℃ Atmosphere:air,95%;carbon dioxide (CO ),5% 2 Subculturing: Protocol: 1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. 3. Add 2.0 to 3.0 ml of Trypsin-EDTA solutionto flask and observe cells under an inverted microscope until cell layer is dispersed. Note:To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting forthe cells to detach.Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 4. Add 6.0 to 8.0 ml of complete growth