Genetic differences among ‘Luotian-tianshi’ (Diospyros kaki Thunb.) genotypes.pdfVIP

Scientia Horticulturae 137 (2012) 75–80 Contents lists available at SciVerse ScienceDirect Scientia Horticulturae j o u rn a l h o me p a ge : w w w. elsev /locate/scih orti Genetic differences among ‘Luotian-tianshi’ (Diospyros kaki Thunb.) genotypes native to China revealed by ISSR and IRAP markers Luxia Yuan, Qinglin Zhang, Dayong Guo, Zhengrong Luo ∗ Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan 430070, China a r t i c l e i n f o a b s t r a c t Article history: ‘Luotian-tianshi’ (Diospyros kaki Thunb.), which has been geographically distributed only in Dabieshan Received 7 August 2011 Mountain around the junction of Hubei, Henan and Anhui Province in central China, is believed to be the Received in revised form 17 January 2012 first PCNA (pollination-constant non-astringent) persimmon native to China. An informative scenario Accepted 20 January 2012 about the genetic diversity and phylogenetic relationships of this native PCNA cultivar is still lacking. To understand the genetic diversity, we have effectively utilized ISSR and IRAP markers to predict the genetic Keywords: differences among 43 ‘Luotian-tianshi’ genotypes collected from three major producing areas in China. Diospyros kaki Thunb. ‘Luotian-tianshi’ A total number of 280 amplicons were observed ranging between 250 and 2000 bp using ISSR and IRAP ISSR