兔单克隆抗体－了解兔单克隆抗体的精彩.ppt

Confidential Rabbit Monoclonal Antibodies --Better Tools for Better Science Advantages of RabMAb Technology Over Mouse MAb Technology Higher quality High affinity and specificity Broad applications High success rate in meeting customers’ criteria Novel Epitopes Recognition of mouse orthologs with high homology to human antigens Higher throughput Stable hybridomas Multiplex High humanization probability Higher homology to human germline sequence than mouse A in vitro positive proof-of-concept study Rabbits Recognize More Epitopes Supernatant From Rabbit Hybridoma Is Highly Active In Western blot A Random Collection Of RabMAbs Have Extremely High Affinities PathoMAb to Identify Therapeutic MAb and Disease Target MAbs Against Mouse Mammary Tumors Raised From A Single Rabbit ~7000 hybridoma clones generated: 157 of 1152 antibodies are useful for immunohistochemistry 80 antibodies preferentially recognize tumor 57 antibodies react with epithelium cells 2 antibodies bind specific for necrotic areas of tumor 11 antibodies react with stroma 2 antibodies stain cell-cell junctions 5 antibodies detect secreted molecules 10 antibodies react with myoepithelial cells RabMAb To A Novel Ion Channel Tumor Antigen--- identified directly from immunization of cancer tissues A Kidney Specific Antibody Generated by PathoMAb Proof Of Principle For Humanized RabMAb --- from 75% to 95% human sequence Market Demand For RabMAbs Improve The Probability Of Success For Drug Discovery Summary RabMAb is a superior technology platform that is widely accepted by the industry Epitomics enjoys rapid growth in customers for research, diagnostic and therapeutic fields Rabbit MAbs have high affinity; Rabbit hybridomas are stable; The success rate to obtain “desirable” MAbs is high; The throughput can be high. * * Mouse Rabbit Molecular-weight marker Fibronectin Protease digested fibronectin 1 2 3 1 2 3 5 mice and 3 rabbits Western Analysis Polysera Immuni