基质辅助激光解析离子-飞行时间质谱仪在快速鉴定常见革兰阴性杆菌中的应用.docVIP

精品论文 参考文献 基质辅助激光解析离子-飞行时间质谱仪在快速鉴定常见革兰阴性杆菌中的应用 [1]新疆昌吉州奇台县人民医院检验科831800 　　[2]新疆昌吉州人民医院输血科831100 　　[3]新疆昌吉州人民医院检验科831100 　　摘要：目的 了解基质辅助激光解析离子－飞行时间质谱仪（MALDI-TOF-MS, MS）在临床分离革兰阴性杆菌鉴定中的应用，为临床医师正确诊断提供科学依据。方法 回顾性分析2015年4月－2015年10月临床标本经MS鉴定为革兰阴性杆菌，将所得菌质谱峰图与数据库中峰图进行比较，分析得出鉴定结果。结果 MS共鉴定出革兰阴性杆菌834株，肠杆菌科622株共6种，占74.58%，主要为大肠埃希菌及肺炎克雷伯菌，分别占肠杆菌科的39.40%及50.16%；非发酵菌属212株共4种，占25.42%，主要为鲍曼不动杆菌和铜绿假单胞菌，其在非发酵菌属中的构成比分别为49.53%及39.15%，使用MS后能鉴定出临床常见革兰阴性杆菌。结论 MS能快速鉴定出常见革兰阴性杆菌，为临床治疗及鉴别感染病原菌提供了快速的筛选方法。 　　关键词：常见革兰阴性杆菌；基质辅助激光解析离子-飞行时间质谱仪；快速鉴定 　　Performance of matrix - assisted laser desorption/ionozation-time of flight mass spectrometry in rapid identification of gram negative bacilli 　　Abstract:Objective To investigate the performance of matrix - assisted laser desorption/ionozation-time of flight mass spectrometry in rapid identification of gram negative bacilli so as to provide scientific basis for correct clinical diagnosis. Methods The gram negative bacilli that were identified from clinical specimens by the MALDI-TOF mass spectrometry were retrospectively analyzed. The mass spectrometer was placed for the identification after it was dried; the acquired bacterial mass spectral peak figure was compared with the peak figure in the databases, and the results of the identification were analyzed. Results A total of 834 strains of gram-negative bacilli have been identified with the use of MALDI -TOF mass spectrometry , including 622 (74.58%)strains of Enterobacteriaceae, with 6 species involved; the Escherichia coli and Klebsiella pneumoniae were the main parts of Enterobacteriaceae, accounting for 39.40%, 50.16%, respectively; there were 212(25.42%) strains of nonfermentative bacteria (4 species), among which the Baumanii and Pseudomonas aeruginosa were the predominant species, accounting for 49.53%, 39.15%, respectively. Conclusion The MALDI-TOF mass spectrometry can identify a variety of species of gram -negative bacilli, and it may facilitate the clinical treatment an the rapid screening of