姜黄素对前列腺癌PC-3裸鼠移植瘤作用的研究.docVIP

精品论文 参考文献 姜黄素对前列腺癌PC-3裸鼠移植瘤作用的研究 1、昆明医科大学第一附属医院 云南 昆明 650032 2、大理大学附属医院 云南 昆明 650032 基金项目: 本课题受云南省校联合基金资助( 2011FB173) 【摘要】目的：探讨姜黄素对前列腺癌PC-3细胞裸鼠移植瘤的作用机制。方法：通过裸鼠皮下接种前列腺癌PC-3细胞建立人前列腺癌雄激素非依赖性裸鼠移植瘤模型，成瘤后随机分为2组，每组6只。姜黄素组：腹腔注射姜黄素50mg/kg，每天1次，共4 w；对照组：腹腔注射等体积的含6% (体积分数)聚乙二醇和含6% (体积分数)无水乙醇的水溶液。取肿瘤称重，并对瘤组织进行组织病理学检查，免疫组化及western blotting检测FASN、Bax蛋白的表达变化。结果：姜黄素可明显抑制前列腺癌PC-3细胞皮下移植瘤生长，姜黄素使肿瘤组织中脂肪酸合成酶FASN的表达水平降低，而使Bax的表达水平升高。结论：：姜黄素可能通过下调FASN及上调Bax的表达抑制前列腺癌PC-3移植瘤的生长。 【关键词】 姜黄素；前列腺癌；FASN；Bax；移植瘤 【中图分类号】R254【文献标识码】A【文章编号】1001-5213(2016)09-0353-02 ABSTRACT: Objective: To explore the mechanism of curcumin in the human prostate cancer cells PC3 xenografted tumor in nude mice. Methods: Human prostate cancer PC-3 cells cultured in vitro were subcutaneously inoculated into nude mice to establish transplanted tumor model. All tumor-bearing mice were randomly divided into 2 groups, there was 6 nude mice in each group: curcumin group, daily intraperitoneal injected(25mgmiddot;kg),a total of 4 weeks. Control group: intraperitoneal injection volume containing 6% (by ??volume) aqueous solution of polyethylene glycol containing 6% (volume fraction) ethanol. The tumor weights inhibition rate were calculated, H amp;E stain was done to examine the difference in histopathology between control team and administ curcumin group team, The expression of FASN、Bax in tumor tissue was determined with immunohistochemical and western blotting methods. Results: Curcumin can inhibit prostate cancer PC-3 cells to tumor growth, obviously． The curcumin decreased the expressionof FASN and increased the expression of Bax in tumor tissues. Conclusion:Curcumin may inhibit the growth of prostate cancer PC-3 xenograft tumor by down-regulating fatty acid synthase (FASN) and increase the expression of Bax. Key words : Curcumin; prostate cancer; FASN; Bax; Xenograft tumor 前列腺癌( PCa)在欧美等发达国家和地区是男性最常见的恶性肿瘤，其死亡率居各种癌症的第二位；在我国前列腺癌发病率低于西方国家，但近年来呈迅速上升趋势。姜黄素（Curcumin）是姜黄的主要活性成分，有较广泛的生物活性，可在体内、外抑制多种肿瘤细胞的增殖并诱导其凋亡[1]。姜