电针对非酒精性脂肪肝大鼠炎症及脂质代谢的影响-第三军医大学学报.DOCVIP

电针对非酒精性脂肪肝大鼠炎症及脂质代谢的影响 朱丽丽，朱艺，苟正江，魏文茂，卓廉士(400016 重庆，重庆医科大学袁家岗校区研究生院行政4班) [摘要]目的:通过观察部分炎症指标及血脂指标，探讨针刺在治疗非酒精性脂肪肝（NAFLD）中对炎症因子及脂肪细胞因子影响的作用机制。方法: 将44只SD大鼠随机分为模型组33只及空白对照组11只。模型组喂养高糖高脂饲料5周制备NAFLD模型，NAFLD大鼠模型建立成功后，将NAFLD大鼠随机分为模型对照组、电针组和非穴组，电针组分别针刺“丰隆”“足三里”“太冲”“三阴交”四个穴位，非穴组电针大鼠尾巴中三分之一段随机四处，避开尾静脉。“治疗”4周后处死所有动物取材，检测血清肿瘤坏死因子-a(TNF-a)、白介素-6 (IL-6)、内毒素（ET）、谷胱甘肽-过氧化物酶（GSH-PX）、脂联素（ADP）、游离脂肪酸（FFA）。结果:电针4周后，电针组与模型对照组相比较，TNF-a、IL-6、ET和FFA下降（P0.05），GSH-PX，ADP上升（P0.05），肝组织内脂肪性变减轻；非穴组与模型对照组变化不明显（p0.05）,肝组织内脂肪性变无明显改善。结论：电针穴位对NAFLD大鼠有较好的治疗作用, 其作用机制可能是通过降低血清TNF-a、IL-6、ET和FFA，升高血清GSH-PX，ADP，减轻肝脏负荷，从而改善肝脏功能实现的。 [关键词]　电针　非酒精性脂肪肝　肿瘤坏死因子-a-6 脂联素 Influence of Electroacupuncture on Serum inflammation and lipid metabolism in Nonalcoholic Fatty Liver Rats Zhu LI-li, Zhu Yi, Gou Zheng-jiang, Wei Wen-mao, Zhuo Lian-shi ( School of Chinese Medicine , Chongqing Medical University , Chongqing 400016 , China) 【ABSTRACT】Objective: To investigate the mechanism of electroacupuncture on nonalcoholic fatty liver disease (NAFLD) through the establishment of NAFLD rats model. Method: 44 rats were randomly allocate to 4 groups, each with 11 rats: control group, electroacupuncture (EA) group, EA non-acupoint (NEA) and normal group. EA (2Hz,1.5mv,15min) rats were applied with unilateral “Fenglong” (ST 40), “Zusanli”(ST 36), “Sanyinjiao” (ST 6) and “Taichong” (LR3) once daily for 4 weeks. Serum tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), endotoxin (ET), glutathione-peroxidase (GSH-PX), adiponectin (ADP) and fress fatty acids (FFA) were detected when the treatment was done. Results: Compared with normal control group, levels of Serum TNF-a，，，GSH-PX and FFA in EA group were decreased significantly (P0.05) and fatty degeneration in hepatic tissue was reduced; no significant change in both serum and hepatic tissue was found in the NEA group (P0.05). Conclusions: EA has a positive effect on the reduction of liver load through reducing the levels of serum TNF-a、IL-6、ET 、FFA,and increasing the levels of se