屎肠球菌抗生素耐药性分析-中国病原生物学杂志.DOCVIP

临床分离的屎肠球菌对8种抗生素的耐药性分析 陈勇生1 黄源春1 1汕头大学医学院第一附属医院检验科，汕头，515041 摘要: 目的 检测中国国内部分地区临床分离的39株屎肠球菌对8种抗生素的耐药性，以及屎肠球菌产β-内酰胺酶的情况。方法 采用纸片琼脂扩散法检测其对青霉素、环丙沙星、红霉素、万古霉素、米诺环素、氯霉素、呋喃妥因及高水平庆大霉素的耐药情况；用头孢硝噻吩法检测屎肠球菌产β-内酰胺酶。结果 39株屎肠球菌对抗生素的耐药情况：对红霉素的耐药率最高，达到94.87％，其次是高浓度庆大霉素和青霉素，分别为84.62％和79.48％，环丙沙星的耐药率也达到76.92％，米诺环素为38.46％，而氯霉素则体现出较好的敏感率，其耐药率为17.95％，尚未发现有耐万古霉素及呋喃妥因的菌株，两者耐药率均为0。39株屎肠球菌β-内酰胺酶阳性率为0。结论 临床分离的屎肠球菌多重耐药性严重，尤其是汕头地区分离的细菌，临床应根据药敏试验选择敏感药物进行治疗。 关键词 屎肠球菌， 抗生素，耐药性 Study on the Antibiotic Resistance of Enterococcus faecium Chen Yongsheng, Huang Yuanchun Department of Clinical Laboratory, the First Affiliated Hospital of Shantou University Medical College, Shantou 515041,China Abstract Objective To investigate the resistance to 8 antibiotics and β-lactamase production of 39 strains of Enterococcus faecium isolated from clinical samples in different regions in China. Methods Susceptibility to penicillin, ciprofloxacin, erythromycin, vancomycin, minocycline, chloramphenicol, nitrofurantoin, and high level gentamicin were analyzed by the disc agar diffusion method. β-lactamase production was detected by nitrocefin method. Results Antibiotic resistance resulted in the following rates: erythromycin 94.87% ,high level gentamicin 84.62%, penicillin 79.48%, ciprofloxacin 94.87%, minocycline 38.46% and chloramphenicol 17.95%, respectively. No vancomycin or nitrofurantoin resistant strains were found. Among 39 Enterococcus faecium strains, β-lactamase production was none. Conclusion Multidrug resistance was serious in Enterococcus faecium isolated from clinical samples, especially in the strains isolated from Shantou. Drug selection should base on the antimicrobial susceptibility results in the treatment. Key words: Enterococcus faecium, Antibiotics, Antibiotic resistance 作者简介：陈勇生(1973-)，男，广东汕头人,主管检验技师，主要从事临床检验工作，E-mail：stchenys@163.com. 通讯作者：黄源春，副主任技师，E-mail: ychun_h@126.com. 屎肠球菌是革兰阳性球菌，是D群链球菌——肠球菌属的第二组，为院内感染常见的病原菌，也是重要的条件致病菌。该菌在临床的分离率约5.00～10.00％，可引起脓毒血症、心内膜炎、脑膜炎及泌尿道、腹腔