荧光原位杂交法观察小鼠皮肤组织切片中型无绿藻感染的研究.pdfVIP

9 ·论著· 荧光原位杂交法观察小鼠皮肤组织切片 中型无绿藻感染的研究 康俞莉赵颖章强强 【摘要】目的探讨荧光原位杂交技术用于诊断小鼠皮肤中型无绿藻感染模型的可行性。方法制作 小鼠皮肤中型无绿藻感染模型，制备皮肤组织石蜡切片，用人工合成的探针PZ—probe进行荧光原位杂交检测， 以过碘酸锡夫(PAS)染色、HE染色做阳性对照；取正常及其他真菌感染的皮肤组织石蜡切片做阴性对照，以同 样的方法进行荧光原位杂交检测，将各种检测方法的结果进行对比分析。结果小鼠临床体征、病理检测及 病原体培养结果均证实皮肤无绿藻感染模型建立成功。通过荧光原位杂交法成功检测出了小鼠皮肤中型无绿 藻感染模型皮肤组织中的无绿藻病原体，结果与PAS染色和HE染色一致；而在正常及其他真菌感染的皮肤 组织石蜡切片中均未能检测出无绿藻。结论荧光原位杂交法可以检测出小鼠皮肤中型无绿藻感染模型组 织石蜡切片中的无绿藻病原体。 【关键词】原壁菌属；皮肤；原位杂交，荧光；小鼠 DetectionofProtothecainfectionin sections fluorescencei玎sltu zopfii mouseskintissue byusing YuH．Zhao of hybridizationKang Ying,ZhangQiangqiang．DepartmentDermatology,HuashanHospital．Fudan 200040’Cbina University，ShaJl幽ai Correspondingauthor：ZhangQiangqiang,Email：zhangqq8@163．com Toevaluatethe todetectPrototheeainamousemodelofPrototheca 【Abstract】Objectivefeasibility zopfii The Prototheca cutaneousinfection fluorescenceinsitu (FISH)．Methodsmodelof byusing hybridization zopfii cutaneousinfectionwasestablishedabdominalsubcutaneousinoculationofProto豳eca into20 by zopfiisuspensions were maleBALB／cmice．Sevenafterthe micewere tissue obtained days inoculation，thesacrificed，andspecimens fromabdominalskinand to cultureand SBbjected microscopicexamination。fungalparaffinembedding．APZ—probe was andusedtodetectProtothecain sections FISH．Moreover． artificiallysynthesized paraffin．embeddedbyusing both acid-S