建立一种新型的胰岛素抵抗性2型糖尿病大鼠模型.docVIP

精品论文 参考文献 建立一种新型的胰岛素抵抗性2型糖尿病大鼠模型 吾甫尔?依马尔1 王护国（通讯作者） 阿迪来木?喀米勒2 　　【摘 要】 目的 建立一种与人类发病相似的胰岛素抵抗性2型糖尿病大鼠模型。方法 选取SD大鼠30只，随机分为正常组和实验组，各15只，正常组整个实验过程中灌胃生理盐水2ml/d。实验组以灌胃高脂高糖乳剂2ml/d稳定饲养8周后，每3天腹腔注射小剂量STZ 15mg/kg4次诱发2型糖尿病大鼠模型后，检测空腹血糖、糖耐量、胰岛素、胰岛素敏感指数等糖代谢指标。结果 与正常组相比，实验组空腹血糖，糖耐量实验（OGTT），胰岛素敏感指数（ISI）功等糖代谢指标都有显著差异（P＜0.05）。结论 该模型具有糖代谢紊乱，其模型可视为研究人类胰岛素抵抗性2型糖尿病理想的动物模型。 　　【关键词】 2型糖尿病； 链脲佐菌素； 糖代谢 　　Establishment of a new type of insulin resistance and type 2 diabetic rat model. Wufuer?yimaer1. Wang huguo1.Adila?kamiletal，1. The First Affiliated Hospital， Xinjiang medical university； 2.The Second 　　Affiliated Hospital，Urumqi Xinjiang 830011 .China; Xinjiang medical university. ) 　　【Abstract】 Objective To establishment of a similar disease in humans insulin resistancein type 2 diabetic rat model.Methods Select the SD rats 30 were randomly divided into normal and experimental groups, each 15, the normal group throughout the experiment intragastric saline 2ml / d. The experimental group received intragastric fat emulsion 2ml / d stable for 8 weeks, every 3 days by intraperitoneal injection of STZ 15mg/kg4 induced type 2 diabetic rat model, respectively, using optical microscopy and transmission electron microscopy the morphology of the pancreatic tissue changes and ultrastructural changes and fasting glucose, impaired glucose tolerance, insulin, insulin sensitivity index and glucose metabolism indicators.Results Compared with normal group, the experimental group, fasting blood glucose, the glucose metabolism of glucose tolerance test (OGTT), insulin sensitivity index (ISI), power and other indicators have a significant difference (P lt;0.05), compared with the normal group, the damage to the pancreas of the experimental grouppathological changes in a significant increase in ultrastructural changes of thedifferences are significant. Conclusion The model has a disorder of glucose metabolism, and pancreatic tissue morphology and ultrastructure of the variation of the model can b