香猪肌肉组织表达序列标签ESTs的分析.pdfVIP

260口中国动物遗传育种研究进展 AssociationofPCR-RFLP ofIGF2Gene Polymorphisms Partial witll GrowthTraitsinDifferent Pigs Liu HuXJaochtm Xin蛳Qishtm Jun HuangshengqiangJjallg and (吩BreedingGenetics，HutumAgricultural University，‰瞥k，410128) asthemaineffect of IGF2 candidate andcarcasstraitsin gene growth Abstract：Using gene PCR—RFLPtOcheckthe inNcirestriction genotypel locus，the variationofIGF2 weredetectedin528 genetic gene 7 the and wemcounted tothePCR— including pigs groups．thengenotypefrequenciesgenefrequencies according RFLPthe of and difference betwecnwere Chi Test．Atthesame frequencies by genolype groups analyzed Square time，the eflh_,tm1 traitsofIGF2 Wasestimated totheir data．PCR-RFLP genetic growth gene accordinggrowth analy— sisshowedthatIGF2aaisfasterthan崛F2”onthe traitsin growth Duroc，I#mdraceaMYorkshire，it’seffectWas Ahasthe 4．06％～8．31％，allele effecton positive growth speed． words：IGF2 Key CANe；PCR—RFLP；Grow山Traits：PertinenceAnalysis 香猪肌肉组织表达序列标签(ESTs)的分析① 王秀利1，2，②李宁1，3，@仇雪梅2李长绿3吴常信3 (1．中国农业大学农业生物技术国家重点实验室，北京，l(】0【)94)