马立克氏病病毒CVI988株病毒囊膜糖蛋白B基因启动子的克隆、及活性分析.pdfVIP

中国畜牧兽医学会禽病学分会禽流感及免疫抑制病论坛论文集 马立克氏病病毒CVl988株病毒囊膜糖蛋白B 基因启动子的克隆、及活陛分析 邱亚峰葛菲菲徐学清陈溥言t+ (南京农业大学动物疫病诊断与免疫重点实验室210095) 摘要：为了选择适宜的启动子调控外源基因的表达，以改善马立克氏病毒为载体的重组病毒的免疫 析发现克隆的gB启动子与Genbank上发表的MgV 子的31倍，SV40启动子为邸启动子的27倍。本研究为下一步gB启动子的应用奠定了基础。 关键词：马立克氏病毒；启动子；转录；转染 the ofMarek’SdiseasevirusCVl988strainand ofits B(gB)genepromoter analysis Cloningglycoprotein function transcriptional 44 QIU GEFei—FeiXU CHENPu—Yan Ya—Feng Xue—Qing ofAnimalDisease and of (KeyLaboratory DiagnosticImmunology，Ministry Agriculture， NanjingAgricultrueUniversity，Nanjiing，210095，China) Abstract：Tothe oftherecombinantvirusesinchickenswithmammal improveefficiency the that the of the selected clonedthe appropriatepromoterregulatedexpressionforeigngenes．Inpresentstudy,we and the ofCVl988 its functional．Thecouldbe transcriptional glycoproteinB(gB)genepromoter analysized study as clonethe and itwiththe in summarizedfollows．(i)we gBpromotercompared sequences toconfermits clonedthe into constructedthe function；(iii)we pSK·-gB--LacZ transcriptional gBpromoter the