南北苍术的RAPD分析及其划分的初步探讨.PDFVIP

南北苍术的 RAPD 分析及其划分 的初步探讨 郭兰萍 ,黄璐琦 , 王　敏 , 冯学峰 , 付桂芳 , 阎玉凝 [摘要] 　目的 :用 RAPD 方法在分子水平上对南北苍术的划分进行初步探讨。方法 :用 40 个引物对 7 个居群的南苍术、北苍术及关苍术进行 RAPD 分析。RAPD 分析软件 RAPDistance Package ———Version 1. 04 中Dice 的方法计算任意两个样品间的遗传距离 ,得树系图。结果 :共检测到多态位点 60 个。聚类分 析显示 ,苍术有以地域为界聚类的倾向 ,且南苍术首先与关苍术聚类后再与北苍术聚类。结论 :苍术的化 学成分、遗传分化和地理分布有一定的相关性 , 以地域为界划分南北苍术有一定道理。 [ 关键词] 　道地药材 ;南苍术 ;北苍术 ;关苍术 ;RAPD A Preliminary Study on Relationship Bet ween Atractylodes lancea and A . chinensis as Analyzed by RAPD GUO L anpin g , HUA N G L uqi , W A N G M in , FEN G X uef en g , FU Guif an g , YA N Y uning [ Abstract] 　Objective : To study the relationship between A . lancea and A . chinensis by RAPD analysis. Method : Using 10 random primers , RAPD of seven A t ractylodes samples was performed. The result was analyzed by RAPD is tance Package Version 1. 04. Result : A total of 60 polymerphic loci were amplified. It appears that the A t ractylodes samples tend to cluster in relation to their habitats ,and A . lancea has closephylo genetic relationship with A . ja ponica . Conclusion : Acertain correlation among the chemical composition , genetic diversity and geographic distribution has been revealed. It is reasonable to separate A . lancea from A . chinensis according to their habitats. [ Key words] 　 authentic medicinal herbs ; A t ractylodes lancea ; A . chinensis ; A . ja ponica ; RAPD ( 　　苍术是常用药材 ,功效燥湿健脾 ,祛风散寒 , 明目。《中国药典》规定其来源于茅苍术 即 ) ( ) ( ) 南苍术 A t ractylodes lancea Thunb. DC. 及北苍术 A . chinensis DC. Koidz. 的根茎。 对南北苍术的划