外源褪黑素促进牛卵母细胞体外成熟的研究.docVIP

高超, 田秀芝, 张璐, 徐静, 汪锋, 卓志勇, 戴蕴平, 刘国世*.外源褪黑素对卵母细胞体外成熟的影响. 中国农业科学, 2011,44（17）:3634-3640 外源褪黑素促进牛卵母细胞体外成熟的研究 高 超, 田秀芝刘国世探索处理对放射免疫分析法P）、E2)、FSH）、LH）水平的变化并检测卵母细胞的活力和活性氧(ROS)的浓度。【结果】牛卵泡液中存在MT浓度与血清中相近，添加10-11 mol·L组的浓度2.01±0.33照组P0.05）。与对照组相比10-9mol·L-1 MT组卵裂率显著提高77.81±7.06% 比vs67.32±7.30%,P0.05），并能提高囊胚率且囊胚细胞数显著高于对照组（90.30±28.74vs75.07±25.98P0.05）10-9 mol·L-1 MT组卵母细胞的活力高于对照组，并能降低卵母细胞内的ROS含量但没有显著差异P0.05）。【结论】牛卵泡液中存在MT，适当浓度的外源MT能促进牛卵母细胞体外成熟。 关键词：褪黑素；卵母细胞；体外成熟；活性氧；牛 Study on the Effect of Exogenous Melatonin ( MT) in Bovine Oocyte in vitro Maturation GAO Chao, TIAN Xiu-zhi, ZHANG Lu, XU Jing, WANG Feng, ZHUO Zhi-yong, DAI Yun-ping, LIU Guo-shi （College of Animal Science and Technology, China Agricultural University, Beijing ) Abstract：mmature oocytes were cultured in vitro inmaturation medium supplemented with different concentrations of?melatonin ,?after IVF statistical analysis embryo?cleavage rate,?blastocyst?rate and?blastocyst cell number?. Detect hormone levels? by radioimmunoassay?in follicular fluid?and the maturation??medium and oocyte viability and reactive oxygen?(ROS)?levels.【Result】MT?exists in bovine follicular?fluid?and similar with serum?. In 10-11 mol·L-1?treatment the progesterone?(P)?levels?2.01 ± 0.33a (P0.05?) of maturation?medium was significantly higher than that of controls .?Compared with the control group?(67.32 ±7.30%), 10-9 mol·L-1MT treatment??cleavage?rate (77.81 ± 7.06%)was significantly?increased?(P0.05)?and improve?the blastocyst rate?and cell number?of blastocyst?was significantly higher than that of controls?(90.30 ± 28.74vs75.07 ± 25.98, P0.05); 10-9 mol·L-1 MT treatment can improve oocyte viability and decrease the ROS level (P0.05). 【Conclusion】Melatonin exists in bovine follicular fluid and exogenous melatonin at the proper concentration can improve the in vitro maturation of bovine oocytes . Key words: melatonin; oocyte; in vitro maturation; ROS; bovine 0 引言 【研究意义】牛卵母细胞体外成熟是体外受精、性别控制、转基因动物生产和动物克隆等技术开展的前提和关键。通过在培养液中添加激素、细胞因子或生长因子等