HPLC法检测黄瓜及土壤中多菌灵的残留量.PDF

山地 农业 生物学报 30( 2 ) : 16 1~ 164, 20 11 Journ al of M ounta in A gricu lture and B io logy HPLC* * 高 洁, 胡德禹, 黄荣茂 ( , , 550025) : 研究了适应于黄瓜和土壤样品中多菌灵残留量测定的高效 相色谱分析方法样品经丙酮/水= 7 / 3( V / V) 提取, 在调节 pH 值的条件下, 用乙酸乙酯萃取净化, 反萃取后浓缩, 高效 相色谱( DAD 检测器测定) , 紫外检 测波长 280 nm该方法的平均回收率为 78 50% ~ 90 01% , 变异系数 4 4% ~ 7 5% , 最低检出限为 0 05 mg / kg 方法的精密度和准确度符合农药残留分析的要求 : 多菌灵; 残留; 黄瓜; HPLC : O 657 72; TQ450 26 : A : 1008- 0457 ( 2011) 02- 016 1- 04 Determination of Carbendazim Residue inCucumber and SoilUsingHPLCMethod * GA O J ie, H UD eyu, H UAN G R ong mao (Cen terf or R esea rch and D eve opm en t of F ine Ch em ica s, K ey L abo ra tory of G reen P esticid e and Ag ricu tura B ioeng ineering of Educa tion M in is try, Gu izhou Un iversity, Cuiyang Gu izhou 550025, Ch ina) Abstract:A n HPLC techn ique w as d eveloped for determ in ing residu e of carbendaz mi in cucumb er and so il in the presen t study T he samp les w ere p icked up w ith acetone /w ater= 7 /3( V /V) and pu rified w ith ethyl ace tate Th e aqueou s so lu tion w as extracted by ethy l acetate in p roper pH A fter concen tration, the target ingre d ien t w as separated by HPLC and detected by DAD detector at 280 nm T he average recovery of the m ethod w as from 78 50% to 90 0 1% , w ith th e coefficient of variation rang ing b etw een 4 40% and 7 50% Th e low est lmi it of detection w as 0 05m g /kg The p recis ion and accu racy of th is m ethod m igh t m eet th e requ ire m en ts o f pesticide residue analys is Key ords: carbendaz mi ; residu e; cu cumber; H PLC ( c