高效液相色谱法测定有机磷原药中有害物治螟磷含量.docxVIP

,,,.[J]., 2014, 53(10): 729-731.高效液相色谱法测定有机磷原药中有害物治螟磷含量崔郭勤 1a，徐远金 1b，罗常泉 2，莫宇星 3(1.广西大学 a.化学化工学院；b.亚热带生物资源保护与利用重点实验室，南宁 530004；2.广西南宁启航环保科技有限公司，南宁 530007；3.广西田园生化股份有限公司，南宁 530007)摘要：[目的]建立有机磷原药中治螟磷高效液相色谱分析方法。[方法]选用Amethyst silica色谱柱，正己烷-四氢呋喃(体积比99.5颐0.5)作流动相，流速为1.0 mL/min，紫外检测波长210 nm，进行外标法定量。[结果]该方法的线性相关系数为0.999 8，标准偏差在0.01～0.02之间，变异系数在0.49%～2.60%之间，平均回收率为99.4%。[结论]该方 法简便快捷，准确度高，线性相关性好。关键词：有机磷原药；治螟磷；高效液相色谱；定量分析中图分类号：TQ450.7文献标志码：A文章编号：1006-0413(2014)10-0729-03Determination of Harmful Substance Sulfotep in TechnicalOrganophosphorous Pesticide by HPLCCUI Guo-qin1a, XU Yuan-jin1b, LUO Chang-quan2, MO Yu-xing3(1.Guangxi University a.College of Chemistry and Chemical Engineering; b.State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Nanning 530004, China; 2.Guangxi Nanning Qi-hang Environmental Proteciton Science and Technology Co., Ltd., Nanning 530007, China; 3.Guangxi Tianyuan Biochemistry Co., Ltd., Nanning 530007, China)[Aims] The HPLC method for determination of sulfotep in technical organophosphorus pesticide wasestablished. [Methods] The sample was separated and analyzed quantitatively with external standard method by Amethyst silica chromatography column at 30 ℃ with a UV-detector at 210 nm using n-hexane and tetrahydrofuran (THF) (99.5颐0.5, by vol) as mobile phase at a flow rate of 1.0 mL/min. [Results] The linear relationship coefficient was0.999 8 with the standard deviation was between 0.01 and 0.02. In addition, the variation coefficient ranged from 0.49 to 2.60% with the average recovery of 99.4% . [Conclusions] The method is simple, rapid and accurate, which has good linear correlation.technical organophosphorous pesticide; sulfotep; high performance liquid chromatography (HPLC);quantitative analysis治螟 磷(sulfotep)，化学名 称 为 四 氧 乙 基二 硫 代 焦 磷酸酯，一种具有触杀、内吸和熏蒸作用的有机磷杀虫剂， 剧毒。其化学性质稳定，常温下不易分解。有机磷农药脂 溶性强，进入 机体 内可分布 到各个 组织 器官 中，对人体 危害极大[1-2]。联合国粮农组织(FAO)、欧盟(EU)及世界卫生组织(WHO)在农药杂质限量表中对治螟磷限量有明确规定