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厌氧氨氧化菌混培物保藏方法的研究 Preservation of ANAMMOX bacteria
中国环境科学2013,33(8):1474-1482
厌氧氨氧化菌混培物保藏方法的研究
汪彩华m,郑 平r,蔡
限公司,北京100176;3-浙江工商大学环境工程系,浙江杭州310018)
(mISO。为保藏基质的方法保藏厌氧氨氧化菌混培物最经济;在保藏试验的5个月内,3种保藏方法下的厌氧氨氧化活性均先快速下降后
趋于稳定,趋于稳定时的活性保留率分别为75.3%,66.3%和47.3%;恶劣的生存环境下,胞外多聚物不仅可维持厌氧氨氧化菌混培物颗粒结
构的稳定性,还可使菌群聚集成团从而增大菌群密度以减少代谢过程中的能量损失;无外界颜色干扰时,厌氧氨氧化菌混培物颜色由血红素
c含量决定,且与活性直接相关.
关键词:厌氧氨氧化菌混培物;温度;基质种类;保藏性能
中图分类号:X703.5 文献标识码:A 文章编号:1000-6923(2013)08—1474-09
ofANAMMoX of
Preservation bacteria.WANG
Cat-hua“,ZHENGJin93,CHEN
Pingr,CAI
Environmental TiandirenEnvironmentand
University,Hangzhou310029,China;2.Beijing
Engineering,Zhejiang
ofEnvironmental
Technology,Corporation,limited,Beijing100176,China;3.Department
GongshangUniversity,Hangzhou
anaerobic methodson
Abstract:Underconditions.three ofANA^n10Xbacteriawere were
preservation studied,which
at4C as natural andat4Cwith 4
with(NI-h):S04matrix,at with(Nl-hhS04
storage temperature
themosteffectivemethod.butatambient themost
℃with(NH4)2804was temperaturewith(NH4hS04was
the5-month the
method.During activitieswith threemethods atthe
preservations,ANAMMOX decayedrapidly
stabilized lateron.Whenactivities survivalactivitieswiththethreemethods
beginning,andgradually gonesteady,the
were not maintainedthestructural
75.3%,66.3%and of
47.3%,respectively.Extracellularpolymerson
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