读书报告6_BT棉花对根际土壤酶活性和土壤微生物的影响.pptVIP

Effect of Bt Cotton on Enzymes Activity and Microorganisms in Rhizosphere Outline 1 Introduction 2 Materials and methods 2.1 Field preparation and cultural practices 2.2 Soil Sampling 2.3 Dehydrogenase 、Alkaline phosphatase 、Urease 、Nitrate reductase activity 2.4 Nematodes（线虫）、Collembola（弹尾目） and Ants 3 Results and discussion 1 Introduction In India nine million hectares (m ha) area was under cotton cultivation and it is one fourth of the global area under cotton cultivation . Bacillus thuringiensis (Bt) cotton was introduced in India in 2002. Following its success, during the last 7 years (2002-2008), the area under Bt cotton has increased by 7.6 m ha from 0.029 m ha . 在印度棉花的种植面积达九百万公顷，占全球棉花种植面积的四分之一。印度在2002年引进苏云金芽孢杆菌(Bt)棉。在过去的7年(2002 - 2008) ，种植面积从29万公顷增加到760万公顷。 James,2008 2 Materials and methods 2.1 Field preparation and cultural practices Field experiments were conducted at the research farm of the Indian Agricultural Research Institute, New Delhi,India. 田间试验布置在印度的农业研究所的农场、新德里、印度。 Experiments were conducted for three consecutive years (2004, 2005 and 2006) in a large plot(450 m2) where Bt and non Bt cotton had ever planted. This plot was subdivided into 24 subplots (3.75m x 5m). 在常年种植Bt和非Bt棉花的土壤上进行了三年(04、05、06年)的连续试验，总面积为450m2，分为24个小区(3.75m×5m)。 Treated :seeds of non Bt cotton and Bt cotton. Distance between row to row and plant to plant :60 cm and 45 cm respectively. Buffer zone : A 6 m also established surrounding the main plot. Recommended dose of NPK fertilizer :660 g N as Urea, 250 g P as SSP, 350 g K as MOP Applied time:phosphorus and Potash was applied at time of sowing, whereas nitrogen was given in split dose, half at the time of thinning and other half at flower