焦磷酸测序定量检测宫颈组织LMX1A基因甲基化水平的临床意义.docVIP

精品论文 参考文献 焦磷酸测序定量检测宫颈组织LMX1A基因甲基化水平的临床意义 王立锋1 朱昊平2 夏子茵1 徐 军1通讯作者 　　1.上海市闵行区中心医院妇产科 上海 201199； 　　2.上海市计划生育科学研究所 上海 200032 　　【摘 要】目的：检测LIM同源盒转录因子1ɑ基因(LMX1A)基因在不同程度病变组中宫颈组织中的甲基化水平，评估其作为宫颈上皮细胞癌变的分子标志物的诊断价值。方法：使用焦磷酸测序法检测每个抑癌基因的甲基化情况。比较正常组织和不同程度病变组织的甲基化水平的的差异。采用利用受试者工作特性曲线（ROC）确定癌变组与非癌变组之间的判别临界值。结果：宫颈癌中的LMX1A基因6个位点的甲基化平均水平为14.36%, 明显高于HG-CIN病变 的4.70%、LG-CIN病变的5.05%和NLM的4.53% (Plt;0.01)。检测LMX1A基因甲基化甲基化鉴别宫颈癌的ROC曲线下面积分别为0.603，敏感度与特异度之和的最大值所对应的界定值为10.66%，诊断浸润性宫颈癌的的敏感度为37%、特异度95%。结论：定量分析宫颈组织中LMX1A基因甲基化将有可能作为分子标志物用于浸润癌的鉴别中。 　　【关键词】LIM同源盒转录因子1ɑ基因；DNA甲基化；宫颈癌；焦磷酸测序法 　　【中图分类号】R737. 33 【文献标识码】B 【文章编号】1674-8999（2015）8-0243-02 　　Quantitative analysis of LMX1A methylation for differentiation on cervical cancers and precursors 　　Wang Lifng 1 ,Zhu Haoping2, Xia Ziyin1, XU Jun1*, 　　 (1. Department of Obstetrics amp; Gynecology, Central Hospital of Minhang District, Shanghai,201199,China) 　　(2. Shanghai Institute of Planned Parenthood Research,Shanghai,200032,China) 　　Correspondence to:Xu Jun E-mail:xujun1958@ 　　Funded by: Shanghai Health Bureau Project 　　【Abstract】Objective：To estimate a quantitative measure of LMX1A methylation in detection of cervical cancer. Methods：The percentage of LMX1A methylation was tested by pyrosequencing in different level of lesions. The efficacy of LMX1A in diagnosis of cercical cancer was tested by ROC curve. Results：The mean methylation value of the LMX1A gene in ICC were 14.36%, significantly higher than that in HG-CIN (4.70%), LG-CIN (5.05%) and NLM (4.53%) (Plt;0.01). The area under the ROC curve (AUC) was 0.603 for LMX1A gene methylation The optimal cut-off value for LMX1A gene methylation was set at 10.66% with the sensitivity of 37% and specificity of 95%. Conclusion：Quatitative measurement of LMX1A by pyrosequencing in cervical caner scraping is highly sensitive and it may have a potential value for diagnosis of cervical cancer in clinic. 　　【Key-words】LMX1A; DNA methylation; Cervical cancer; Pyrosequencing 　　宫颈癌的发病率和死亡率分