猫爪草总皂苷对荷瘤小鼠抑瘤作用的实验研究.docVIP

精品论文 参考文献 猫爪草总皂苷对荷瘤小鼠抑瘤作用的实验研究 吴殿斌 陶萍（辽宁大连大学附属中山医院 116000） 　　【摘要】目的 观察猫爪草总皂苷对荷瘤小鼠的抑瘤作用、免疫功能和生存时间的影响。方法 将制作好的荷瘤小鼠分为模型组、CTX组、猫爪草总皂苷高剂量组和低剂量组，观察猫爪草总皂苷对Lewis肺癌荷瘤小鼠的抑瘤率、测定NK细胞和T细胞亚群的数量，计算荷瘤小鼠的生存率。结果 猫爪草总皂苷对荷瘤小鼠有一定的抑瘤作用，高剂量组的抑瘤率为47.27%，低剂量组的抑瘤率为31.63%；猫爪草总皂苷能提高荷瘤小鼠的NK细胞活性，高、低剂量的NK细胞数量均较模型组升高，并能提高荷瘤小鼠的CD4细胞数量，显著提高生存率。结论 猫爪草总皂苷具有一定的抑瘤作用，提高荷瘤小鼠的免疫功能和生存率。 　　【关键词】猫爪草 荷瘤小鼠 抑瘤作用 　　【中图分类号】R73 【文献标识码】A 【文章编号】1672-5085（2013）49-0007-02 　　【Abstrac】Objectives: The objective of this paper was to investigate the effectsof total saponins from Radix ranunculus ternati on anti-cancer,immune function and surviving time in a sample of tumor bearing mice.Methods: The pre-prepared tumor bearing mice were divided to control group and CTX group, high dose total saponins from Radix ranunculusternati group and low dose total saponins from Radix ranunculus ternati group, then the effects of total saponins from Radix ranunculus ternati on anti cancer effects, the number of NK cell and T cell sub group in tumor bearing mice were evaluated, to understandtheir survival rate.Results: Radix ranunculus ternati has certain effects on anti cancertreatment, the findings from this study indicate it was 47.27% in thehigh dose group and 31.63% in low dose group; Polysaccharides from Radix ranunculus ternati group can increase the activity of NK cell,particularly in high dose group and low dose group compared to thecontrol group; it can also increase the number of CD4 cells whichsignificantly led to a higher survival rate.Conclusions: Radix ranunculus ternati has certain effects on anticancer treatment, and also enhance the immune function and survivalrate. 　　【KEYWORDS】 Radix ranunculus ternati, tumor bearing mice, anti cancer effects 　　猫爪草Radix ranunculus ternati为毛莨科植物小毛莨Ranunculus ternatusThunb的块根，因其块根肉质，数个簇生，近纺锤形，外皮黄褐色，形似猫爪而得名，主产河南、江苏、广西、安徽、江西等省区[1]，具有化痰散结解毒消肿之功效; 用于瘰疬痰核，疔疮肿毒，蛇虫咬伤等病症[2]，猫爪草通过调节免疫作用而具有较好的抗菌、抗炎、抗肿瘤等作用[3-4]。有文献报道，猫爪草对恶性肿瘤如乳腺癌有良好