谷氨酸棒杆菌分批发酵生物絮凝剂的动力学模型研究.pdf

谷氨酸棒杆菌分批发酵生物絮凝剂的动力学模型 安仲涛，陈仁彬，江新德，李清彪，杨坤，何宁* （厦门大学化学化工学院化学工程与生物工程系，福建厦门361005） 摘要:对谷氨酸棒状杆菌生产生物絮凝剂的分批发酵过程进行了动力学研究。以 Logistic 方 程来描述菌体生长过程，并对 Gaden 相关模型加入时间修正因子进行修正，建立了产物生成 动力学模型。提出了葡萄糖和尿素两个基质消耗动力学模型。通过 mathematica 软件对实验 数据进行拟合，显示各模型都能较好地描述谷氨酸棒杆菌合成生物絮凝剂的发酵过程。为谷 氨酸棒杆菌合成生物絮凝剂分批发酵过程的进一步优化控制提供指导。 关键词: 谷氨酸棒状杆菌; 生物絮凝剂;分批发酵; 动力学 中图分类号： Q 文献标识码：A 联系人：何宁. 第一作者：安仲涛（1984—）， Corresponding author: He Ning. E-mail: 男，硕士研究生. hening@xmu.edu.cn 基金项目：国家自然科学基金 Foundation item: supported by the National Natural Science Foundation of China ） Kinetic models for bioflocculant production by Corynebacterium glutamicum AN Zhongtao, CHEN Renbin, JIANG Xinde, LI Qingbiao, YANG Kun, HE Ning (Department of Chemical and biochemical engineering，School of Chemistry and Chemical Engineering, Xiamen University ，Xiamen 361005 ，Fujian ，China) Abstract: Kinetic models to represent the process kinetics for batch fermentation of bioflocculant by Corynebacterium glutamicum were reported in this paper. A series of mathematical models were hypothesized and proved. Logistic equation was applied to describe the process of cell growth, and Gaden relevant model with time-corrected coefficient was established to illustrate bioflocculant synthesis. Two matrix kinetic models represented the consumption of substrates. Curve fitting was done with the software Mathematica and results showed that the models could well describe the batch cultivation process of bioflocculant production by Corynebacterium glutamicum, thus providing guidance for further process optimization of flocculant biosynthesi