甘草多糖体外抗癌活性及对移植瘤小鼠免疫功能的.docVIP

精品论文 参考文献 甘草多糖体外抗癌活性及对移植瘤小鼠免疫功能的 1.商丘医学高等专科学校 药理学教研室 河南 商丘 476100 ；2商丘医学高等专科学校 病理学教研室 河南 商丘 476100 ；3商丘医学高等专科学校 医学检验教研室 河南 商丘 476100 【摘要】目的：分析甘草多糖体外抗癌活性及对移植瘤小鼠免疫功能的影响。方法：体外培养肿瘤细胞，观察甘草多糖对肿瘤细胞生长的抑制作用；采取随机数字表法将50只小鼠随机分成5组，每组10只，甘草多糖3个剂量组为150、300、600mg/kg体重，阳性对照组为200 mg/kg体重，阴性对照组给予生理盐水，对小鼠的胸腺、肿瘤组织和脾脏进行称重，计算胸腺指数、脾脏指数，抑瘤率。结果：体外抗肿瘤结果，甘草多糖各剂量组IgM、IgG含量、抗体生成细胞水平明显高于阴性、阳性对照组, 其中大剂量组差异显著(P﹤0.05),一定剂量的甘草多糖能显著抑制肿瘤细胞的生长，且剂量过高会影响其抑瘤效果。结论：甘草多糖具有较强的抗癌活性及对移植瘤小鼠免疫功能具有较好的生物活性，值得参考。 Abstract Objective: To analyze Glycyrrhiza Polysaccharide in vitro anticancer activity and its effect on immune function in mice transplanted tumor. METHODS: Cultured tumor cells, the inhibition observed Glycyrrhiza Polysaccharide on tumor cell growth; to take a random number table 50 mice were randomly divided into five groups of 10, three doses of licorice polysaccharide group 150,300,600mg / kg body weight, the positive control group was 200 mg / kg body weight, the negative control group received normal saline on the thymus of mice, tumor tissue and spleen were weighed, thymus and spleen indexes, the inhibition rate. Results: In vitro anti-tumor results, licorice polysaccharide in each dose group IgM, IgG content, antibody-producing cells was significantly higher than the negative, the positive control group, in which the high-dose group were significantly different (P lt;0.05), licorice polysaccharide certain dose significantly inhibited growth of tumor cells, and the dose is too high will affect the anti-tumor effect. Conclusion: Glycyrrhiza polysaccharide has strong anti-cancer activity and immune function in mice transplanted tumor has good biological activity, it is also useful. 【关键词】甘草多糖；体外抗癌活性；移植瘤小鼠；免疫功能 【中图分类号】R282.77【文献标识码】A【文章编号】1001-5213(2016)08-0328-01 甘草也被称为甜草根，具有缓急止痛、润肺止咳、泻火解毒、缓和药性的功效,主要的成分包括了三萜、黄酮、多糖类化合物[1]。依据相关的研究说明多糖有抗肿瘤、抗病毒、抗炎、降血糖、抗凝血等相关的生物活性。本实验从提取黄酮后的甘草残渣中提取分离甘草多糖，探讨了甘草多糖体外抗癌活性及对移植瘤小鼠免疫功能的影响，现将体会报道如下。 1.资料与方法 1.1实验资料 1.1.1 主要试剂及实验药品 主要试