生地与熟地降糖降脂作用比较研究.docVIP

精品论文 参考文献 生地与熟地降糖降脂作用比较研究 浙江常山县人民医院，常山 324200 摘要 目的 观察比较生地黄、熟地黄对糖尿病小鼠糖脂代谢的影响，为中医糖尿病防治的处方遣药提供药理依据。方法 以高糖高脂喂养加四氧嘧啶静脉注射构建糖尿病小鼠模型，以血糖和血脂为主要指标，观察比较生地黄和熟地黄对模型小鼠的降糖降脂功效。结果 对模型小鼠的降糖降脂作用，生地黄比熟地黄强。结论 中医运用中药地黄防治糖尿病时，以生地黄入药较为适宜。 关键词 生地黄；熟地黄；糖尿病；糖脂代谢 【中图分类号】R614.2【文献标识码】A【文章编号】1001-5213(2016)08-0090-02 Comparative Study of Radix Rehmanniae and Rehmannia Glutinosa on the Glucose and Lipid Metabolism in Diabetic MiceDiabetes with Yin Deficiency ABSTRACT: OBJECTIVE Observing and comparing the effect of Radix rehmanniae and Rehmannia glutinosa on the glucose and lipid metabolism in diabetic mice, provide the pharmacological basis for the prescription of traditional Chinese medicine preventing and curing diabetes. METHOD mice models with DYBS were reproduced by high fat and high sugar diet for 30 days, than injected 50mg/kg Alloxan in tail vein. with the main index of blood glucose and lipids levels, observe and compare the anti-diabetes and anti-hyperlipidenmia efficacy of Radix rehmanniae and Rehmannia glutinosa in model mice. RESULTS Comparing the anti-diabetes and anti-hyperlipidenmia efficacy in diabetic mice, Radix rehmannia is better than Rehmannia glutinosa. CONCLUSION When using traditional Chinese medicine rehmannia to prevent and cure diabetes, Radix rehmanniae is more suitable. KEY WORDS：Radix rehmanniae; Rehmannia glutinosa; diabetes; glucose and lipid 地黄为玄参科多年生草本植物地黄Rehmannia glutinosa Libibosch.的新鲜或干燥块根，始载于《神农本草经》，具有滋阴清热、补血止血等功效，临床应用极广，中医常取其滋阴清热之效用于糖尿病的治疗。一般认为，生地黄（简称生地）甘寒，有清热凉血、养阴生津之功；熟地黄（简称熟地）甘温，有滋阴补血，益精填髓之效。在古今糖尿病防治的中医处方中，生地黄与熟地黄均有入药，且比例相当。为了弄清生地与熟地究竟哪个用于糖尿病治疗更为合理，实验以此前复制成功的阴虚血瘀型糖尿病小鼠模型[1]为平台，对二者的降糖降脂作用进行了比较研究，现将结果报道如下： 1 材料与方法 1．1 生地与熟地样品，从市场上购得生地黄，经鉴定系玄参科植物地黄的干燥块根。洗净，闷润，切厚片，干燥得生地。取上述生地，按每100g生地药材加40g黄酒拌匀，装入高压锅中隔水炖至黄酒被吸尽，乌黑色光泽，味转甜，取出，晒至外皮粘液稍干时，切厚片，干燥即得熟地。将生地与熟地分别打成细粉，临用时用蒸馏水分别配制成同样浓度（含生药量）的混悬液备用。养阴降糖片，正大青春宝药业有限公司产品，批所有药物的给药剂量以g（生药）/kg（体重）计。 1．2 动物，ICR小鼠，清洁级，20plusmn;2g，全部♂，由浙江省医学科学院实验动物中心提供。合格证号：S