复方阿苯达唑伊维菌素及其单药的体内外致突变性研究.PDFVIP

DOI ：10.7524/AJE.1673-5897.20160923002 邓林, 龚玲,杨家书,等. 复方阿苯达唑/伊维菌素及其单药的体内外致突变性研究[J]. 生态毒 理学报, 12(5): Deng L, Gong L, Yang J S, et al. The studies on the mutagenic effects of the combination and its components of albendazole and ivermectin in vivo and vitro[J]. Asian Journal of Ecotoxicology, 12(5): (in Chinese) 复方阿苯达唑/伊维菌素及其单药的体内外致突变性研究 邓林，龚玲，杨家书，赖诗韵，陈阳，林居纯* 四川农业大学动物医学院，四川成都 611130 收稿日期：2016-09-23 录用日期：2017-02-04 摘要：农业生产上常用的阿苯达唑和伊维菌素均有一定的致突变性。为了解伊维菌素和阿苯 达唑按 1:24 形成固定剂量复方的致突变性，以及复方和单药致突变性的差异，采用 Ames 试验，小鼠骨髓微核试验和小鼠精子畸变试验进行复方阿苯达唑/伊维菌素及其单药的致突 变性研究。结果表明，复方阿苯达唑/伊维菌素能提高小鼠骨髓嗜多染红细胞的微核率和精 子畸形率，但在单复方的比较中发现，单药的微核率和精子畸形率均高于复方，表明复方毒 性有所降低。同时，无论是复方还是单药均不能提高组氨酸缺陷型沙门氏菌的突变率。以上 表明，虽然复方阿苯达唑/伊维菌素致突变毒性低于单药，但仍然是一种能引起小鼠骨髓微 核率和精子畸形率升高的致突变阳性药物，因此需警惕该药物的食品残留问题及对生态环境 的危害。 关键词：复方阿苯达唑/伊维菌素；单药；致突变 文章编号：中图分类号：文献标识码：A The Studies on the Mutagenic Effects of the Combination and its Components of Albendazole and Ivermectin in vivo and vitro 1 1 1 1 1 * Deng Lin , Gong Lin , Yang Jiashu , Lai Shiyun , Chen Yang , LinJuchun College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China 基金项目：2016 年质量工程-大学生创新创业训练计划 作者简介：邓林(1986-) ，男，在读硕士研究生，研究方向为新兽药安全性评价，E-mail: denglin1514@163.com *通讯作者(Corresponding author)，E-mail: juchunlin@126.com Received 23 September 2016 accepted4 February2017 Abstract: Albendazole and ivermectin, common antiparasitic drugs widely used in livestock production, are showed mutagenic effects in some extent. The mutagenic effects of the combination (1:24) and its components of ivermectin and albendazole were investigated by utilizing Ames test, mouse bone marrow micronucleus test and mouse sperm aberration test. We found that the micronucleus frequency of bone marrow and the frequency of sperm abnormality were increased in mice treated with the combination. In comparison with the