BMPRBMFS复合物修复关节软骨全层缺损的实验研讨.pdf

哈尔滨医科大学硕士研究生论文 中 文 摘 要 摘「要〕目的:探讨骨形态发生蛋白/自体红骨髓/纤维蛋白凝胶 (BMP/RBM/FS)复 合物修复关节软骨全层缺损的效果。方法:采用健康成年日本大耳白兔27只，随机分 成A,B、C三组 (每组18个关节) 在每只兔双侧股骨的骸股关节面中心各造成一直 径为4咖 深3。的全层关节软骨缺损。A组关节缺损内植入BMP/RBM/FS复合物，B组关 节缺损内植入FS凝胶与RBM混合物，C组为空白对照组，不做任何处理。术后4,8,12 周分批处死动物，取材，对修复组织进行大体、组织学和扫描电镜观察。结果:A组 组织修复快，质量好，术后4周缺损区完全填充，12周修复组织与周围正常软骨组织 的外观相近，光镜下见近乎正常关节软骨组织，扫描电镜下见形态成熟的透明软骨细 胞和11型胶原。B组和C组的修复质量差。结论:BMP/RBM/FS复合物能促进和改善关 节软骨的修复，并能以透明软骨修复全层关节软骨缺损。 [关键词〕关节 软骨缺损 骨形态发生蛋白 自体红骨髓 纤维蛋白凝胶 载体 哈尔滨医科大学硕士研究生论文 ABSTRACT ANEXPERIMENTALSTUDYONREPAIRINGOFFULL-THICKNESS DEFECTSOFARTICULARCARTILAGEWITHACOMPOUNDOF BONEMORPHOGENETICPROTEIN REDBONEMORROW ANDFIBRINSEALANT ObjectiveTostudytheeffectsofbonemorphogeneticprotein,redbonemorrow andfibrinsealant(BMP/RBM/FS)onthehealingoffull-thicknessdefectsofarticular cartilage.Methodstwentysevenadultrabbitswererandomlydividedintothree groups,groupA,B,C(eaehgroupwitheighteenarticulations),Onefull-thicknessarticular cartilagedefectof4;M indiameterand3mm depthwascreatedthroughthearticular cartilageinthecenterofeachfemoropatellararticularsurface.ThedefectsofgroupAand BwerefilledwithBMP/RBM/FSandFS/RBMrespectively.ThedefectsofgroupCwere leftempty.Thehealingofthedefectswasobservedbygrossandmicroscopicexamination at4,8and12weeksafteroperation.Theshapeofchondrocytesandcollageninthedefects wereexaminedwithscreeningelectronmicroscope(SEM). Results Therepairing processingroupAwasfasterandbetterthanin groupBandgroupC.Attheendofthe fourthweek,newtissuefilledareaofdefects.Attheendofthetwelthweeksthegross appearanceofthedefectswassimilartothatofnormalcartilagearoundthedefects. Histologicalappearanceofthedefectswasidenticaltothatofnormalarticularcartilage. Maturebylinechondrocytesandtype11collagenwerefoundwithSEM.Conclusion Thecompound