长角血蜱和微小扇头蜱的形态与分子生物学鉴定-中华流行病学杂志.PDFVIP

·实验室研究· 长角血蜱和微小扇头蜱的形态 与分子生物学鉴定 覃新程田俊华王剑波卢昕孙强正金东周敦金徐建国张永振 【摘要】 目的建立鉴定长角血蜱和微小扇头蜱的形态学和分子生物学相结合的方法。 方法采集自湖北和河南省布尼亚新病毒疫区家养动物体表寄生的蜱及草丛、灌木中的蜱；先以 形态学鉴定，后用PCR方法扩增得到蜱的12S rDNA，测序后进行同源性和系统进化分析。结果 形态学方法鉴定采集到两种蜱：长角血蜱(Haemaphysalislongicornu)和微小扇头蜱(Rhipicephalus rDNA经克隆、测序，用PAUP microplus)。蜱的12S 4．0软件构建系统发生树，湖北、河南省两种蜱 的12S rDNA序列分别与长角血蜱和微小扇头蜱聚类，与形态学鉴定结果～致。结论在传统形 态学分类的基础上结合分子生物学鉴定方法能更准确地鉴定蜱的种类。 【关键词】长角血蜱；微小扇头蜱；鉴定 Identificationof and HaemaphysalisIongicornisRh扣icephalusmicroplusQINXin—cheng。，T1AN Jun一^∽2，WANGjid卅b01，LuXin。，SUN Qiang-zhen91，J，ⅣDonFl，ZHOUDun-fin2，xuJian-guo。， zHANG for ControlandPrevention．National Yong-zheaj、StateKeyLaboratorylnfectiou』Disease InstituteforCommunicableDiseaseControlandPrevention．∞ineseCenterDiseaseControland for DiseaseControland Prevention，＆讲w102206，China；2∥“^an Prevention Centerfor com Correspondingauthor：ZHANGYong-zhen，Email：yongzhenzhang@sohu Toestablishamethodcombined andmolecularmarker 【Abstract】0bjective morphology for and MethotisTickswerecollected identifyingHaemaphysalislongicornisRhipicephalu^microplus fromdomesticanimalsandwildenvironmenti11 areaofHubeiandHenan where epidemic provinces casesoffevetwith were Weclassifiedtheticks thrombocytopeniasyndromeprevalent by rDNA characteristicsbefore12S oftickswere PCRand morphology amplifiedby subsequently was bv 0 Results to tre