柳树Saliz babylonica L叶浸提液对蛋白核小球藻Chlorella pyrenoidosa的化感作用研究.pdfVIP

柳树(Salix babylonica L)叶浸提液对蛋白核小球藻 （Chlorella pyrenoidosa）的化感作用8 李庆华，郭沛涌*，田美燕，江中央 （华侨大学环境科学与工程系，福建泉州 362021 ） 摘 要 通过对蛋白核小球藻进行细胞密度的测定，阐明了不同浓度柳树叶浸提液对蛋白核小球藻生长和细 胞形态的影响。结果表明，柳树叶浸提液对蛋白核小球藻具有化感作用，作用 96 小时的半效应浓度(EC50,96h -1 -1 -1 值)为 11.82 g·L ，但不同浓度的浸提液化感作用强度不同。较低浓度(10 g·L 和 20 g·L )浸提液对蛋白核小球 -1 -1 -1 藻表现出先抑制后促进的作用特性，而较高浓度（30 g·L ）浸提液则始终表现出抑制效果。10 g·L 、20 g·L 和 30 g·L-1 浸提液对蛋白核小球藻的最大抑制率分别为 37.5%、39.3%和 56.5% 。柳树叶浸提液作用下，部分 蛋白核小球藻细胞平均颗粒体积增大，颜色变透明，膨胀后溶解。低浸提液浓度处理组少数细胞聚集在一起， 高浓度浸提液处理组细胞大量聚集，形成多细胞群体，多数沉于瓶底，沉降性能增强。 关键词 柳树叶浸提液；蛋白核小球藻；化感作用 中图分类号： 文献标识码：A 文章编号： Allelopathy of the Extract of Salix babylonica leaves on Chlorella pyrenoidosa Li Qing-hua, GUO Peiyong * , TIAN Mei-yan, JIANG Zhong-yang (Department of Environmental Science and Engineering, Huaqiao University) Abstract Laboratory experiments were investigated to elucidate the allelopathic effects of extract from Salix babylonica L leaves on the growth and morphology of the green alga Chlorella pyrenoidosa by mensurating the algae density. The results show that the extract of Salix babylonica L leaves had allelopathic effect on Chlorella pyrenoidosa, the effects concentration of 96 hours is 11.82 g·L-1, but the effect varied with different concentration. The extract with low concentration (10 g·L-1 and 20 g·L-1) showed inhibition at first and then turned to accelerate to the alga’s growth. However, the extract with high concentration (30 g·L-1) showed inhibiting effect all the time in the experiment. The highest inhibitory rate are 37.5%, 39.3% and 56.5%, respective