全氟辛烷磺酸PFOS对斑马鱼生理生化指标的影响研究1袁璐瑶1.DOCVIP

全氟辛烷磺酸（PFOS）对斑马鱼生理生化指标的影响研究 袁璐瑶1，潘若雷1，江敏1,2 （1.上海海洋大学 水产与生命学院，上海 201306；2.上海市水域环境生态上海高校工程研究中心，上海 201306 摘要：为探讨全氟辛烷磺酸（PFOS）对鱼类的毒性效应，参考PFOS对斑马鱼(Danio rerio)的96h-LC50值，设置了0、1、5和10 mg/L 4个浓度组，采用静水式实验测定了PFOS对斑马鱼头部碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）和乙酰胆碱酯酶（AchE），内脏团中ALP、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和丙二醛（MDA）含量以及肌肉中ALP、SOD、MDA含量。结果表明：斑马鱼头部ALP和AchE活力会在实验初期短时间内受到抑制，之后逐渐恢复；中低浓度PFOS（1 mg/L和5 mg/L）胁迫会使得头部LDH活力显著升高。内脏团中ALP活力受到显著诱导，并与PFOS浓度呈一定的剂量-效应关系；SOD和CAT活性则在0.5~1 h受到显著诱导，之后有所下降，但并未受到显著抑制；MDA含量在PFOS胁迫下显著升高。肌肉中的ALP活力受到显著抑制（P＜0.05），SOD活力及MDA含量受PFOS影响不显著。 研究亮点:目前，PFOS对斑马鱼的毒性研究多集中于对斑马鱼鳃、胚胎、肝脏及基因损伤的影响，但仅从这几方面探究PFOS对斑马鱼的毒性具有一定的局限性。本文通过对斑马鱼头部、内脏团及肌肉中ALP、AchE、LDH、SOD、CAT这几种酶及MDA含量的监测，综合评定PFOS对斑马鱼的毒性效应，为更全面地了解PFOS的水生生态毒性提供理论依据。 关键词：毒性效应；全氟辛烷磺酸； 斑马鱼； 酶活性；抑制；诱导 Effects of PFOS on the physiological and biochemical Indicies of Danio rerio YUAN Luyao1, PAN Ruolei1 , JANG Min1,2 （1 College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; 2 Research and Engineering Center on Aquatic Environment Ecosystem, Shanghai 201306, China） Abstract: Referring to the 96h-LC50 values of Perfluorooctane sulfonate (PFOS) on Danio rerio, four groups with concentration of 0, 1, 5 and 10mg/L were set to explore the toxic effect of PFOS　on fish． Indices of the physiological and biochemical parameters, such as the activities of alkaline phosphatase(ALP), lactate dehydrogenase(LDH), and acetylcholinesterase (AchE) in the fish head, the superoxide dismutase(SOD), catalase(CAT), malondialdehyde (MDA) and ALP in the fish visceral were analyzed. ALP, SOD and MDA in the muscle of Danio rerio were also determined. The results showed that the activities of ALP and AchE in the head would be inhibited at the beginning of the experiment, then recover gradually. PFOS with medium-low concentration(1mg/L and 5mg/L）forced LDH activity in the head induced remarkably. ALP in the visceral mass was significantly induced and showed dose-effect relationship with PFOS concentration. After being significantly induced at