西瓜种质资源的遗传多样性及亲缘关系的AFLP分析.PDFVIP

西瓜种质资源的遗传多样性及亲缘关系的AFLP分析.PDF

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西瓜种质资源的遗传多样性及亲缘关系的AFLP分析

华北农学报2007, 22( 增刊) : 177180 AFLP 李艳梅, 段会军, 马峙英 ( , 071001) : AFLP 57 DNA , 18AFLP 709DNA , 39. 4, 440,61.4%; DNA ,0.24~ 0. 99, 0.70, NTSYS ,57 211, 2 2 , ,,AFLP , , :; ;; ;AFLP :S65101 :A : 1000- 7091( 2007) - 0177- 04 Study on Genetic Diversity and Relationship in Watermelon Germplasms by AFLP Analysis LIYan ei,DUAN Huijun,MA Zhiying (College of Agrono y,AgriculturalUniversity of Hebei,Baoding 071001,China) Abstract : 57water elon aterials wereanalyzed usingAFLP.The results showed that 709 AFLP bandswere a pli fied using 18pri er pairs,a ong the ,440(61.4%)bands werepoly orphic.Based on DNA bands,the si ilarity coeffi cient were calculated and their averagevaluewas 0.70,ranging fro 0. 24 to 099,which indicated that relationshipwas close and their geneticbasewasnarrow a ong aterials of water elons.When allof the poly orphicbandswere analyzed by cluster analysis, 57 water elon aterials were classified into 2groups.Group included 1wildwater elon aterial,and group,56water elon aterials fell into it.A ong the group introduced water elon aterials were clustered together as well as theF1and parent aterials.In addition,this study de onstrates that AFLP fingerprintinghas higher resolution and can reveal the difference of cultivars,so it is an efficient tool. Key words: Water elon; Cultivar; Geneticdiversity; Relationship; AFLP analysis [ 2] 3%Zhang X P 3, 1 RAPD , 53 5 , , 623%,3 [3] 101% Lee S J 39 RAPD , [ 4] Toshi

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