香菇子实体蛋白多糖的分离纯化及组成结构分析.pdfVIP

植物资源与环境学报 2001,10(2):4一6 JournalofPlantResourcesandErwironmeru 香菇子实体蛋白多糖的分离纯化及组成结构分析 杨 娟，，吴谋成2，张声华2 (l贵州省·中国科学院天然产物化学重点实验室，贵州贵阳550002;2.华中农业大学食品科技系，湖北武汉430070) 摘要:香菇(Lend=edodes(Beck.)Sing.)子实体经热水提取，乙醉沉淀，得多搪粗品(I})oLe脱游离蛋白，脱色，经 DEAF.纤维素柱及纤维索凝胶柱层析分离纯化得到I}l,le-2和L}33个级分。经聚丙烯陇胺凝胶电泳和钊- CL-6B柱层析鉴定为分子量分布均一的蛋白多塘。凝胶渗透色谱法洲得1-1,1--2和Le-3分子It分别为954000, 90000和14000o3个级分均由阿拉伯糖、木糖、甘阵锗、半乳精、荀萄褚和葡萄箱醛酸组成，气相色谱洲得I-1的阿 拉伯抢、木错、甘璐精、半乳特、蔺萄特的康尔比为。.39:0.46:1.00:0.93:14.13;1rr2为。.19:0.41:1.00:0.93: 10.72;1a-3为0.31:0.47:1.00:1.15:8.920Le-1,1}2和1-3葡萄糖酸酸含量(%)分别为24.10,34.77和40.05,蛋 白质含C%)分别为2.01,7.38和25.31，红外扫描确定Le-1和Ic2的精昔键为。型，Le-3为P型。 关扭词:香菇;蛋白多精;分离;纯化;性质 中圈分炎号:5567.39;Q539 文橄标识码:A 文章幼号2001)02-0004-03 Isolation,ptiflcadnoandcLeraderizadonofpolysaccharidesfromtheflddx吻 吐LentLtttsedodes (Bark.)Snig.YANGJuari，WUMou-chenj，MANGSheng-huat(1.TheKeyLaboratoryofChemistry forNaturalProductsofGuizhouProvinceandtheChineseAcademyofSciences,Guiyang550002,China;2. DepartmentofFoodScienceandTechnoloyg,HuazhongAgriculturalUnivresiyt,Wuhan430070,China),J. PlantResourE4riron.2001,10(2):4一6 Abractts: Afterbeingextractedwithhotwaterandprecipitatedwithalcohol,rcudepolysaccharide(Le)was obtainedfromfruitbodyofLentmusedodes(Bark)Sing.Le. removedfireproteinsandcolouringmatter, then isolated and polysaccharides(] SephamseCG6B homogeneousmolecularweights.MolecularweightsofL-1 Le-2andLe-3were954〕】)， 90000and 14000,respectively,场脚permeationhC-ogt a户y.Threefiactions。 composedofarabinose(Ara), xillyulossrteate(Xdythl)a,tmthae豁neutralMsaacnc)h,argidaelascmtooslearr(Gaatilo)o,fgLlue-co1sweasG(Alcr)X::anydlM::gluancumG:nalicG:aclcid=.G0a.s39chm:0m.4a6to:g1r.a0p0h): 0G.9a3lG::14lc.1=3;0L.e3-21二w0:a.4s7Ara:1.X:0y0lM::1a.n15G:a8:l.9G:2lc.=G0lu.cm19on0:i.c4a1ci:d1.c0o0nt0:en.9ts3%(:10).7o2fathnrdeeLefr-a3cwtioanssAwraereX:2y4l..M:10an, 34.77and40