英文flavonoids.ppt

FLAVANOIDS Contents Chapter 1 The chemistry of Flavonoids potent antioxidant compounds that have demonstrated greater antioxidant protection than vitamins C and E, increase the activity of antioxidant enzymes, inhibit cancer by blocking the formation of cancer-causing compounds and suppressing the activation of carcinogens. The major polyphenols in green tea are flavonoids (catechin, epicatechin and so on). Chapter 2 Isolation and Identification of Flavonoids  Chapter 3 Structure Elucidation - Depends on the goals of the conducted research. Avoid the chemical and/or enzymatic degradation of the metabolites. 2.2 Isolation 2.2.1D The extraction procedure for obtaining flavonoid conjugates from biological material Attention ! On the other hand, in the phytochemical analysis of plant species or phytopharmaceutical studies of plant material, the repeatable isolation of all biologically active flavonoid aglycones with a good yield is more important. - Need more drastic extraction conditions. 2.2 Isolation 2.2.1D The extraction procedure for obtaining flavonoid conjugates from biological material a. Robust multistep chromatographic methods - Isolation of individual components from plant extracts containing new uncharacterized compounds. 2.2 Isolation 2.2.1F Isolation of individual components The sequence and kind of separation methods used depends on the composition of the sample and the experience of the researcher. However, minor Flavonoids components are difficult to obtain as pure compounds. ▲ Analysis of samples containing a number of compounds present in small amounts, the application of an analytical chromatographic systems enhanced by proper detectors (UV, NMR, and/or MS) - Gives spectrometric information sufficient for establishing the structure of minor target components. ▲ Liquid chromatography with multiple detector systems (UV diode array d