沙门氏菌志贺氏菌和金黄色葡萄球菌共增菌培养基的研制-食品科学.PDFVIP

※生物工程 食品科学 2013, Vol.34, No.17 171 沙门氏菌、志贺氏菌和金黄色葡萄球菌共增菌 培养基的研制 1 1 2 1,* 1 王永志 ，余以刚 ，黄秀丽 ，肖性龙 ，吴 晖 (1. 510640 2. 516003) 华南理工大学食品安全与检测中心，广东 广州 ； 广东省惠州市质量计量监督检测所，广东 惠州 摘  要：为研制出一种能够同时选择性富集沙门氏菌、志贺氏菌和金黄色葡萄球菌的培养基，对多种抑制剂和促进 剂进行筛选。实验优化出的最佳培养基配方为：胰蛋白胨15g、大豆蛋白胨5g、磷酸二氢钾2.5g 、葡萄糖2.5g 、氯化 钠35g、氯化锂0.5g、牛胆盐0.1g、甘露醇2g 、亚碲酸钾0.3mg，蒸馏水1000mL。沙门氏菌、志贺氏菌和金黄色葡萄 3 7 球菌等目标细菌在此共增菌培养基中均能快速生长，以10 CFU/mL为初始菌浓，18h后菌浓能达到10 CFU/mL ，而 非目标菌在此肉汤中的生长受到抑制。结果表明，此培养基可以用于沙门氏菌、志贺氏菌和金黄色葡萄球菌的选择 性共增菌。 关键词：选择性共增菌；沙门氏菌；志贺氏菌；金黄色葡萄球菌 Salmonella Shigellosis Staphylococcus aureus An Enrichment Broth for Simultaneous Growth of , and WANG Yong-zhi1，YU Yi-gang1，HUANG Xiu-li2 ，XIAO Xing-long1,*，WU Hui1 (1. Detection Centre for Food Safety, South China University of Technology, Guangzhou 510640, China ； 2. Huizhou Quality and Measuring Supervision Testing Institute, Huizhou 516003, China) Abstract ：In order to develop an enrichment broth for the simultaneous growth of Salmonella , Shigellosis and Staphylococcus, several candidate agents were added to pre-formulated broth to observe their growth speeds. The optimal performance was obtained with the enrichment broth composed of 15 g of tryptone, 5 g of soya peptone, 2.5 g of monopotassium phosphate, 2.5 g of glucose, 35 g of sodium chloride, 0.5 g of lithium chloride, 0.1 g of bile salt, 2 g of mannitol and 0.3 g of potassium tellurite in 1000 mL of distilled water. Results