甘草有性生殖特性研究-中国药科大学学报.DOCVIP

甘草花粉超微鉴定及花粉活力、柱头可授性荧光显微镜观察 马春英1，屈平2，王文全3 （1河北农业大学农学院/河北省作物生长调控重点实验室，河北保?定 071001； 2河北农业大学生命学院，河北保定 071001；3北京中医药大学中药学院/中药材规范化生产教育部工程研究中心，北京 100102） 摘要：本文利用扫描电子显微镜对乌拉尔甘草、光果甘草和胀果甘草花粉的超微结构进行观察；利用荧光显微镜确定乌拉尔甘草的授粉方式、花粉生活力和柱头活性以及授粉后受精时间。结果表明花粉超微结构为甘草的鉴别提供了一定的形态学依据；甘草的授粉方式属于闭花受精；花粉生活力在每天12:00时最强，去雄后超过4d的柱头已经不具备接受花粉的能力；对授粉后不同时间的雌蕊进行研究得出授粉后6h花粉管进入胚珠进行受精。本文主要对甘草的有性生殖特性进行了研究，为未来制定正确的选、育种策略提供理论依据。 关键词：甘草；花粉活力；柱头可授性；荧光显微镜 Study on the sexual reproduction properties of the liquorice MA Chun-ying1, QU Ping2, WANG Wen-quan3 (1 Key Laboratory of Regulation and Control of Crop Growth of Hebei Province, College of Agronomy, Agricultural University of Hebei, Baoding 071001, Hebei; 2 School of Life Science, Agricultural University of Hebei, Baoding 071001, Hebei; 3 School of Chinese Pharmacy, Beijing University of Chinese Medicine, the Engineering Research Center for Chinese Medicin Standardized Production of Educational Ministry, Beijing 100102) Abstract: The ultrastructure of the pollen in the Glycyrrhiza uralensis F., G. glabra L., and G. inflata Batal. was observed by the scanning electron microscope (SEM). The pollination way, the pollen viability and the stigma activity, the fertilization time ascertainment after pollination were observed by the fluorescence microscope. The results showed that the study on the ultrastructure of pollen offered certain morphology basis for the discrimination of the liquorice. The pollination way of the liquorice was cleistogamy. The pollen viability was the highest at 12:00 in daytime, and the stigma had no ability to accept the pollen exceeding four days after artificial emasculation. The pollen tube entered into the ovule to fertilize 6h after pollination when the fixed pistils were observed different time after pollination. In order to establish the correct strategy of seed selection and breeding of the liquorice in the future, the sexual reproduction properties of the liquorice was studied in the study. Keywords: liquorice; sexual reprodu