载INHRFP白蛋白纳米粒的制备及体外释药的研究-第三军医大学学报.DOCVIP

载异烟肼、利福平白蛋白纳米粒的制备及其体外释药研究 马 荣1，陈 振1，张惠勇1，杨小英3，王文萍2，戈朝晖4 (750004银川，宁夏医科大学：研究生院1,药学院2；宁夏soniazid,INH)、利福平(Rifampicin,RFP)牛血清白蛋白(Bovine serum albumin,BSA)纳米粒的制备及体外释药特性。方法 以白蛋白为载体聚乳酸为骨架，采用改良自乳化-溶剂扩散法制备纳米粒。采用透射电镜观察纳米粒形态；激光粒度衍射分析仪测定纳米粒粒径分布；高效液相色谱法测定纳米粒的载药量和包封率；动态膜透析法观察载药纳米粒的体外释药特性。结果 制备的INH-RFP-BSA-NPs电镜下观察外观圆整，大小均匀、分散性好，粒径为（60.54.6）nm。异烟肼、利福平的载药量分别为19.8%、20.1%，包封率分别为87.8%、98%。INH至6d时累计释放97.02%，RFP至5d时完全释放。结论 以白蛋白和聚乳酸为载药基质，采用改良自乳化-溶剂扩散法可制备出粒径小、载药量和包封率较高的载异烟肼、利福平白蛋白纳米粒,体外释药显示载异烟肼、利福平白蛋白纳米粒具有良好的缓释作用。 [关键词] 白蛋白；异烟肼；利福平；纳米粒；体外释药 [中图法分类号] [文献标志码] A Studies on preparation and in vitro release of albumin nanoparticles loaded with isoniazid and rifampicin Ma Rong1, Chen Zhen1, Zhang Huiyong1, Yang Xiaoying3, Wang Wenping2, Ge Zhaohui4 (1 Gratuate School ,2 College of Pharmacy,Ningxia Medical University; 3 Department of Pharmacy,4 Department of Orthopedics Sugery, General Hospitalof Ningxia Medical University, Yinchuan, Nixia Hui Autonomous Region ,750004,China) [Abstract] Objective To study the preparation and release characteristics of bovine serum albumin nanoparticles loaded with isoniazid and rifampicin（INH-RFP-BSA-NPs）in vitro. Methods INH-RFP-BSA-NPs were prepared by modified self-emulsion solvent diffusion method,Albumin and Polylactic acid was used as a carrier as well as skeleton structure.The transmission electron microscope was used to observe the morphology of INH-RFP-BSA-NPs.The nanoparticle’s size and it’s distribution of INH-RFP-BSA-NPs ware performed by submicron particle size analyzer.The high performance liquid chromatography (HPLC) method was used to measure the drug loadings and the coating rate of INH-RFP-BSA-NPs. Dynamic membrane dialysis method to study the in vitro release characteristics of INH-RFP-BSA-NPs. Results The INH-RFP-BSA-NPs were smooth , sphere-like, relatively uniform in size and good dispersion. The submicron particle size analyzer showed the diameter was (60.54.6)nm. The drug loading and entrapment efficie