白头翁寒热药性的细胞评价.pdfVIP

Drug Evaluation Research 第 36 卷 第 5 期 2013 年 10 月 - 359 - 白头翁寒热药性的细胞评价 王 征，张 宁，孙艳妮，刘建利* 西北大学生命科学学院 西部资源与现代生物技术教育部重点实验室，陕西 西安 710069 摘 要：目的 通过实验室前期建立的中药寒热药性的细胞评价方法评价中药白头翁的寒热药性。方法 用噻唑蓝（MTT ） 比色法考察白头翁对HeLa 细胞和 SGC-7901 细胞体外增殖的影响，倒置显微镜观察白头翁对细胞形态特征的影响，台盼蓝 染色法分析白头翁的细胞毒作用。结果 白头翁在所选质量浓度 10～400 μg/mL 内抑制细胞增殖。形态学观察表明白头翁能 使细胞密度减小，细胞固缩变圆，颗粒感增加。台盼蓝染色表明白头翁对这两种细胞没有细胞毒作用。结论 白头翁性寒， 对所用细胞没有细胞毒作用。 关键词：白头翁；MTT 法；HeLa 细胞；SGC-7901 细胞；台盼蓝染色 中图分类号：R285.1 文献标志码：A 文章编号：1674 - 6376 (2013) 05 - 0359 - 04 DOI: 10.7501/j.issn.1674-6376.2013.05.009 Evaluation of cold-heat characteristics of Pulsatillae Radix WANG Zheng, ZHANG Ning, SUN Yan-nin, LIU Jian-li Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi’an 710069, China Abstract: Objective To evaluate the cold-heat characteristics of Pulsatillae Radix by the method we have set up. Methods MTT assay was used to investigate the effect of Pulsatillae Radix on the proliferation of HeLa and SGC-7901 cells in vitro. The morphological changes of HeLa and SGC-7901 cells treated with Pulsatillae Radix were observed through inverted microscope. Trypan-blue staining was used to analyze the cytotoxicity of Pulsatillae Radix. Results Pulsatillae Radix could inhibit the proliferation of HeLa and SGC-7901 cells in the concentration range of 10—400 μg/mL. Morphological observation showed Pulsatillae Radix in the concentration range we have chosen could decrease the density of HeLa and SGC-7901 cells and condense the cells. Trypan-blue staining showed Pulsatillae Radix had no cytotoxicity on HeLa and SGC-7901 cells. Conclusion Pulsatillae Radix