轮状病毒NSP4基因变异特征.docVIP

精品论文 参考文献 轮状病毒NSP4基因变异特征 木国法 郭正红 　　【摘 要】 目的 研究轮状病毒流行株NSP4基因变异特征。方法 对2015年7-11月从云南省昭通市获得的6株人轮状病毒流行株的NSP4基因用RT-PCR进行扩增,并进行测序，测序结果应用DNAassist和Clustal-mp以及MEG4.0软件进行序列分析,通过昭通毒株RV NSP4基因与来自GenBank Database的4株人RV (Wa、Hochi、 KUN、AU-1)和3株动物RV(OSU、SAll、 EW)及我国不同地区的45株A组轮状病毒NSP4基因进行比较。结果 昭通地区6株轮状病毒株的NSP4基因都为Wa型,与来自Genbank Database的4株人RV (Wa、Hochi、 KUN、AU-1)和3株动物RV(OSU、SAll、 EW)氨基酸同源性分别为95.7%、、94.2%、86.4%、84.8%和91.2%、82.3%、48.3%,与中国各地区流行株同源性均在86.4%以上, 据此可将51株RV NSP4分为2组,分别以Wa株和KUN株为代表,其中以Wa组为主,组内同源分别为92.6%-100%和96.5%-100%。氨基酸进化树提示在Wa组内包括3个亚组。结论 6个昭通地区轮状病毒株的NSP4基因都为Wa型。我国流行株NSP4基因主要可分为Wa组及KUN组,NSP4的变异与年份有关而与地域关系不密切。 　　【关键词】 轮状病毒； 非结构蛋白4； 腹泻； 患儿 　　Genetic characterization of the Zhaotong, Yunnan rotavirus NSP4 compared with other 45 epidemic strains from China. Guofa Mu Zhenghong Guo. Department of pediatrics, The first Peoplersquo;s Hospital of Zhaotong City, Yunnan ,China 657000 　　【Abstract】 Objective To clarify the features of NSP4 gene variation among epidemic strains of human rotavirus in China. Methods NSP4 genes from 6 epidemic strains of human rotavirus isolated in ZhaoTong City,Yunnan from July to November in 2015 were amplified with RT-PCR. Then NSP4 specimensrsquo; cDNAs were sequenced and analyzed by DNAassist 、Clustal-mp and MEG4.0 software. The 6 specimensrsquo; NSP4 cDNAs sequences were compared with other 45 RV NSP4 cDNAs sequences available from China in the Genebank Datebase to clarify the features of NSP4 gene variation among epidemic strains of human rotavirus in China. Results The NSP4 Genotype of the 6 ZhaoTong RV strains were Wa strains .The amino acid homologies to the 4 HRV standard strains ( Wa、Hochi、 KUN、AU-1) and 3 animal RV( OSU、SAll、 EW)were 95.7%、94.2%、86.4%、84.8% and 91.2%、82.3%、48.3% respectively. Compared with the epidemic strains of other different areas of China, the amino acid homology of the NSP4 gene were over 86.4%. Based on the variation of amino acid sequence the 52 virus strains can be divided into t