辛伐他汀对急性肺栓塞兔炎细胞因子的影响.docVIP

精品论文 参考文献 辛伐他汀对急性肺栓塞兔炎细胞因子的影响 山东省聊城市人民医院 252000 摘要：目的：探讨辛伐他汀对急性肺血栓栓塞症兔血清肿瘤坏死因子-alpha;（TNF-alpha;）及白细胞介素-1beta;（IL-1beta;）的影响。方法 选用30只新西兰健康白兔，随机分为对照组、模型组、辛伐他汀干预组。采用兔自体血栓回输法建立急性肺栓塞模型。采用酶联免疫吸附法检测血清肿瘤坏死因子-alpha;及白细胞介素-1beta;的浓度变化。结果 栓塞后3小时，模型组TNF-alpha;、IL-1beta;浓度（5.52plusmn;0.99ng/ml，183.06plusmn;30.43pg/ml）与对照组（2.16plusmn;0.63ng/ml，94.26plusmn;8.57pg/ml）、辛伐他汀干预组（4.20plusmn;0.88ng/ml，146.09plusmn;21.07 pg/ml）比较有显著统计学意义（plt;0.01，plt;0.01）。栓塞6小时两者浓度基本达基线水平。结论 急性肺血栓栓塞后，血清TNF-alpha;、IL-1beta;浓度升高；辛伐他汀可降低急性肺血栓性栓塞血清TNF-alpha;、IL-1beta;水平。 关键词：动物模型；细胞因子；辛伐他汀 [Abstract]Objective To explore the changes of serum tumor necrosis factoralpha;（TNF-alpha;）interleukin1beta;（IL-1beta;）and effect of simvastatin on acute pulmonary thromboembolism（APTE）rabbits.Method APTE models were established by injecting autologous blood clots into right atrium by femoral vein.30 healthy zelanian rabbits were selected and divided into the control group（n=10），model group（n=10）and simvastatin group（n=10）randomly.The changes of TNF-alpha; IL-1beta; in the blood serum were tested with an enzyme-linked immunosorbent assay（ELISA）.Result At 3 hour point after embolization，TNF-alpha; IL-1beta; of model group arrived at 5.52plusmn;0.99ng/ml，183.06plusmn;30.43pg/ml which were higher in comparison with those（2.16plusmn;0.63ng/ml，94.26plusmn;8.57pg/ml and 4.20plusmn;0.88ng/ml，146.09plusmn;21.07pg/ml）in control group and simvastatin group.The values of model group were higher in comparison with the control group and the simvastatin group（Plt;0.01，Plt;0.01）.Conclusion After APTE，cytokines of TNF-alpha; IL-1beta; increased obviously.Simvastatin may inhibit the cytokines.After APTE，anti-inflammatory therapy may become an important treatment. [Key words] Animal Model；Cytokines；Simvastatin 本研究通过自体血栓回输法复制肺血栓栓塞动物模型，探讨急性肺血栓栓塞兔血清中TNF-alpha;、IL-1beta;浓度变化及辛伐他汀对其的影响。 1资料与方法 1.1 动物分组 健康新西兰白兔30只（山东大学实验动物中心提供，许可证号：SCX，雌雄不限，体重（3.2plusmn;0.3）kg，月龄4－6个月，随机分为对照组、模型组、辛伐他汀组，每组10只。制模前治疗