【生物学论文】植物DNA甲基化与作物种质资源保存.pdf

20 10, 11( 6) : 659665 Journa l o f P lant G enetic R esources 植物 DNA 甲基化与作物种质资源保存 毛新国, 李 昂, 景蕊莲 ( / / , 100081) : DNA 甲基化是真核生物基因表达调控的重要机制之一甲基化 DNA 与甲基特异 合蛋白 合, 并作为染色质修 饰复合物识别和作用的平台, 参与对染色质组织方式的调节, 最终影响基因的表达本文对植物 DNA 甲基化及其生物学功 能检测分析方法以及作物种质资源保存中存在的甲基化现象进行了综述, 旨在为深入了解 DNA 甲基化修饰对种质资源的影 响, 更好地开展作物种质资源保护供参考 : DNA 甲基化 基因表达调控 逆境胁迫 种质资源保存 Plant DNA M ehtylation and Crop Germplasm Conservation MAO X inguo, L I A ng, JING Ru ilian (NationalKey Facilityf or Crop Gene Resources and Genetic Improvement/Key Laboratoryf or Crop Germp lasm Resources and Utilization, inistry of Agriculture/ Institute of Crop Science, ChineseAcademy of AgriculturalSciences, Beij ing 100081) Abstract: DNA m ethylat ion is one o f the mi portant regulat ion m ech an ism s for gene expression in eukaryotic ge nom e. M ethy lated DNA can interact w ith sp ec ific m e thylbinding prote in, and acts as a p la tfo rm to facilitate the rec ognit ion o f chrom at in com plex, and invo lves in the organ ization of chrom osom e stru cture, w hich ultmi ate ly determ ines gene s expression. H ere, w e rev iew ed the research progress on plant DNA m ethylat ion, summ arized its b io log ica l function s and detect ion m ethods, and introdu ced the w ide occurrence o f DNA m ehty lation, wh ich m ight lead to phe notype variation in the process of crop germp lasm con servation. T he purpose is to provide a clue to understand the DNA m ehty lat ion and exp lore an approach for the security of conserved crop germ p lasm. K eywords: DNA m ethylat ion G ene expression and regulat ion A biotic stress G erm p lasm con servation , ( ep igenet ics) ( genom ic mi printing) ( tran sposon ac , tivation) RNA ( RNA editin