柑橘柠檬苦素大孔树脂分离纯化方法-食品科学.pdfVIP

24 2015, Vol.36, No.14 食品科学 ※工艺技术 柑橘柠檬苦素大孔树脂分离纯化方法 庞 立，邓子牛，刘吉凯，王仁才* （湖南农业大学园艺园林学院，湖南长沙 410128 ） 摘  要：以柑橘柠檬苦素为考察指标，研究大孔树脂分离纯化柠檬苦素的工艺条件。结果表明，D101大孔树脂对 柑橘柠檬苦素有较好的吸附分离性能，是分离纯化柑橘柠檬苦素的适宜大孔树脂；D101大孔树脂分离纯化柑橘柠 檬苦素的最佳工艺条件为：上样流速1 mL/min、上样质量浓度0.5 mg/mL、用pH 6、80%的乙醇溶液作洗脱剂、洗 脱流速2 mL/min。通过树脂回收重复使用，发现D101树脂通过再生处理后，其吸附性能未有明显降低，可以重复 使用。采用上述方法得到D101大孔树脂对柠檬苦素的吸附率为88.53%，解吸率为93.47%，得率为82.75% 。高效液 相色谱检测可知，柠檬苦素的含量达到了92.79%。 关键词：柑橘；柠檬苦素；大孔树脂；分离；纯化 Separation and Purification of Citrus Limonin by Macroporous Resin * PANG Li, DENG Ziniu, LIU Jikai, WANG Rencai (College of Gardening and Horticulture, Hunan Agricultural University, Changsha 410128, China) Abstract: A method for the purification of limonin from citrus by macroporous resin was proposed. The results showed that  D101 resin was the best adsorbent for the purification of citrus limonin. The optimal purification conditions were achieved  by loading the sample containing 0.5 mg/mL limonin onto the column at a flow rate of 1 mL/min and subsequently eluting  the  alcoholatpH6ataflowrateof2mL/min.TheadsorptioncapacityofD101resinremained adsorbed limonin with 80%                                     almostunchanged afterseveralcyclesofrepeateduseandregeneration.Undertheoptimizedconditions,theadsorptionrate,                                 desorption rate and yield of limonin were 88.53%, 93.47% and 82.75%, respectively. The result of HPLC showed that the  limonin content (i.e., purity) of the purified product was 92.79%. Key words: citrus; limonin; D101 macroporous resin; separat